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TranscriptomicsHela Mitotic Selection - gene expression time series in early G1 phase of the human cell cycle
HeLa mitotic cells were collected using a mitotic shakeoff apparatus to study gene expression in early G1 phase of the human cell cycle. This set includes microarray data from two shake-off experiments : shake 1 (0h-14h) which was conducted over 14 hours and RNA samples collected every 2 hrs, and s...
ORGANISM(S): Homo sapiens 
2010-06-25 | E-GEOD-12473 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of cell cycle in HeLa S3 cells, double thymidine block, rxperiment 2
HeLa S3 cells were synchronized in early S phase with a double thymidine block as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 24K arrays for the second double thymidine block synchronization (Thy-Thy2).
ORGANISM(S): Homo sapiens 
2005-09-22 | E-SMDB-902 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of cell cycle in HeLa S3 cells, double thymidine block, experiment 3
HeLa S3 cells were synchronized in early S phase with a double thymidine block as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 43K arrays for the third double thymidine block synchronization (Thy-Thy3).
ORGANISM(S): Homo sapiens 
2005-09-22 | E-SMDB-903 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of cell cycle in HeLa S3 cells, double thymidine Block, experiment 1
HeLa S3 cells were synchronized in early S phase with a double thymidine block as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 24K arrays for the first double thymidine block synchronization (Thy-Thy1).
ORGANISM(S): Homo sapiens 
2005-09-22 | E-SMDB-901 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of cell cycle in HeLa S3 cells, thymidine-nocodazole lock
HeLa S3 cells were synchronized in mitosis by arresting first in S phase with thymidine followed by arrest in mitosis with Nocodazole as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 43K arrays for the thymidine-nocodazole synchronization (Thy-Noc)...
ORGANISM(S): Homo sapiens 
2005-09-22 | E-SMDB-904 | biostudies-arrayexpress
TranscriptomicsA multi-protein occupancy map of the mRNP on the 3' end of histone mRNAs
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Homo sapiens 
2015-12-16 | E-GEOD-62154 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of cell cycle in HeLa S3, mitotic selection, experiment 1
HeLa S3 cells in mitosis were selected by mitotic shake-off using an automated cell shaker (Eliassen et al. 2000, MCB)by Beth Alexander and Myra Hurt at Florida State University. This experiment set contains the complete set of 43K arrays for the first mitotic shake-off experiment described in Whit...
ORGANISM(S): Homo sapiens 
2005-09-22 | E-SMDB-905 | biostudies-arrayexpress
TranscriptomicsAnti-SLBP RIPchip data
The animal replication-dependent (RD) histone mRNAs are coordinately regulated with chromosome replication. They are the only known cellular mRNAs that are not polyadenylated. Instead, the mature transcripts end in at a conserved stem-loop (SL) structure. This SL structure interacts stably with the...
ORGANISM(S): Homo sapiens 
2015-12-16 | E-GEOD-59915 | biostudies-arrayexpress
TranscriptomicsScleroderma Architecture Cell Lines
The data from these cell lines is described in figure 2 of the mansucript "Systemic and cell-type specific gene expression patterns in scleroderam skin" by Whitfield et al. (2003) PNAS. Aortic smooth muscle cells and microvascular endothelial cells were obtained from Clonetics and culture...
ORGANISM(S): Homo sapiens 
2005-12-20 | E-GEOD-3887 | biostudies-arrayexpress
GenomicsLive cell monitoring of periodic gene expression in synchronous human cells identifies forkhead genes involved in cell cycle control
We report the genome wide DNA binding patterns of wild type FOXK1 in asynchronous HeLa cells using chromatin precipitation followed by high-throughput sequencing (ChIP-seq). We find that FOXK1 is bound to the promoter of a number of cell cycle genes including the E2F binding partner TFDP1. This st...
ORGANISM(S): Homo sapiens 
2012-07-09 | E-GEOD-39138 | biostudies-arrayexpress
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