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TranscriptomicsGlobal Deterministic and Stochastic Allelic Specific Gene Expression in Single Blastomeres of Mouse Early Embryos
Here we modified a single cell whole transcriptome amplification method to make it capable of amplifying cDNAs as long as 3kb efficiently and unbiasedly. We combined this modified single cell cDNA amplification method with Applied Biosystems next generation sequencing SOLiD™ System to set up a singl...
ORGANISM(S): Mus musculus 
2010-12-31 | E-GEOD-22182 | biostudies-arrayexpress
ProteomicsZebrafish early embryos isolated nuclei and whole blastomeres LC-MSMS
Nuclear pore complex (NPC) structure and maturity changed during early development before MBT in zebrafish embryos. Aiming to further confirm the difference of NPCs among different stages during MZT, we first isolated nuclei from 32-cell and 512-cell stage embryos as most zygotic genes have not been...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2023-08-25 | PXD017896 | Pride
Whole blastomeres Lc-msms Isolated nuclei Zebrafish 
TranscriptomicsComparative analysis of gene expression profiles between four kinds of blastomere at 8-cell stage embryos.
The establishment of body axes and specification of early embryonic cells depend on maternally supplied transcripts and/or proteins, several of which are localized at specific regions of fertilized eggs and early embryos. The ascidian is known to exhibit a mosaic mode of development, and this mode ...
ORGANISM(S): Ciona intestinalis 
2010-06-19 | E-GEOD-15637 | biostudies-arrayexpress
TranscriptomicsThe identification of differentially expressed genes between animal and vegetal blastomeres in Xenopus laevis
To identify asymmetrically localized maternal mRNAs along the animal-vegetal axis in cleavage Xenopus embryos, we isolated animal and vegetal blastomeres at 8-cell stage, extracted the maternal mRNA respectively and analyzed them by RNA-seq technology. We identified 43 maternal transcripts significa...
ORGANISM(S): Xenopus laevis 
2015-05-19 | E-GEOD-68972 | biostudies-arrayexpress
TranscriptomicsDefining Cell Fate And Embryonic Genome Activation By Global Single-Cell cDNA Analysis of Blastomeres From 5-to 8-Cell Human Embryos.
To determine blastomere fate and embryonic genome activation (EGA) at 5- to 8-cell stage human embryos by global gene expression profile of amplified cDNA from blastomeres at the single cell level Forty-nine blastomeres from 5-, 6- and 8-cell human embryos were analyzed through whole genome wide ana...
ORGANISM(S): Homo sapiens 
2010-10-30 | E-GEOD-22032 | biostudies-arrayexpress
TranscriptomicsComparison of animal-blastomeres' RNA content to vegetal-most half of vegetal blastomeres' RNA content at the 8-cell stage of Xenopus laevis embryos to identify animal-enriched transcripts
In Xenopus laevis, a number of studies identified vegetal factors that specify the germ line, endoderm and dorsal axis, but there are few studies demonstrating roles for animal-enriched maternal mRNAs. Therefore, we carried out a microarray analysis to identify novel maternal transcripts enriched in...
ORGANISM(S): Xenopus laevis 
2013-12-21 | E-GEOD-48659 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA-seq of blastomeres from 2- to 32-cell stage mouse embryos
Transcriptomes were determined for all blastomeres of 28 embryos at the 2- (n=8), 4- (n=16) and 8-cell (n=4) stages, and for individual cells taken from 16- (n=6) and 32- (n=6) cell stage embryos. We also carefully monitored the 2- to 4-cell divisions noting whether the cleavage plane was meridional...
ORGANISM(S): Mus musculus 
2016-03-23 | E-MTAB-3321 | biostudies-arrayexpress
Transcriptomicsarray-CGH on single blastomeres from day-3 human cleavage stage embryos
Seventy blastomeres from fourteen frozen-thawed supernumerary human preimplantation embryos were disassociated and genomic was amplified using Multiple Displacement Amplification. BAC array-CGH was performed on the amplified products. BAC array-CGH on single blastomeres amplified by Multiple Displac...
ORGANISM(S): Homo sapiens 
2012-10-15 | E-GEOD-34290 | biostudies-arrayexpress
UnknownOrigin and development of uniparental and polyploid blastomeres.
Not available
S-EPMC12020880 | biostudies-literature
TranscriptomicsGene expression profiles of individual blastomeres of early Ciona embryos
We examined gene expression profiles of individual blastomeres of early Ciona embryos using microarrays. We examined gene expression profiles of individual blastomeres of the 8-cell, 16-cell and 32-cell embryos by dissecting with a fine glass needle. We also examined gene expression profiles of unfe...
ORGANISM(S): Ciona intestinalis 
2013-10-29 | E-GEOD-45548 | biostudies-arrayexpress
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