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With the goal of identifying changes in gene expression in CD4(+) T cells during the development of diabetes in the nonobese diabetic (NOD) mouse, we used DNA microarrays to analyze gene expression in CD4(+) T cells from the pancreatic draining lymph nodes of NOD/BDC 2.5 T cell receptor transgenic a...
ORGANISM(S): Mus musculus 
We followed the Arabidopsis tape-sandwich protocol to isolate the leaf protoplast enriched with palisade mesophyll cells. Briefly, control and WD-stressed Arabidopsis rosette leaves were placed on 3M tape, and abaxial cell layers were peeled off by gently removing the tape, thereby exposing the adax...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-06-18 | MSV000095072 | MassIVE
Single-cell proteomic data from RO-3306 treated alveolar type II epithelial cells (C10) and untreated C10 cells. Cells were sorted using cellenONE onto several nanoSPLITS chips. Cell lysates were then split for parallel RNAseq and proteomic analysis. For proteomics recovery experiment where both chi...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-12-02 | MSV000090828 | MassIVE
Human pancreatic islets from two human donors (Prodo Laboratories) were acquired and cultured for 24 hr in pancreatic islet media (PIMS, Prodo Laboratories). Single cells were dissociated with TrypLE for ~15-30 minutes, washed with PBS, stained with Calcein-AM/propidium iodide, and sorted onto nanoS...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-07 | MSV000093330 | MassIVE
WTCCC project samples from the Parkinson's disase cohort
Genome-wide association scan was performed in 1,705 cases of parkinson's genotyped on the Illumina Human 660W SNP array, and 5,175 controls genotyped on the Illumina1.2M-Duo. Analysis was carried out on the overlaps set of SNPs.
To address the protective mechanisms generated in animals on previous exposure to this parasite, using samples of abomasal mucosa collected pre- and post- challenge from animals of differing immune status in an experimental model of Teladorsagia circumcincta infection.
ORGANISM(S): Ovis aries 
To address the protective mechanisms generated in animals on previous exposure to this parasite, using samples of abomasal mucosa collected pre- and post- challenge from animals of differing immune status in an experimental model of T. circumcincta infection.
ORGANISM(S): Ovis aries 
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