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UnknownExtensive Molecular and Clinical Heterogeneity in Patients With Histologically Diagnosed CNS-PNET Treated as a Single Entity: A Report From the Children's Oncology Group Randomized ACNS0332 Trial.
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S-EPMC9153369 | biostudies-literature
TranscriptomicsChanges in cholesterol homeostasis and acute phase response following pulmonary exposure to multi-walled carbon nanotubes links carbon nanotube exposure to risk of cardiovascular disease
Adverse lung effects in rodents following pulmonary exposure to multi-walled carbon nanotubes (MWCNT) are well documented. However, systemic effects are less understood. Prospective epidemiological studies have shown increased cardiovascular disease risk after pulmonary exposure to airborne particle...
ORGANISM(S): Mus musculus 
2015-02-02 | E-GEOD-61366 | biostudies-arrayexpress
ProteomicsMultimodal measurement of the transcriptome and proteome in G2/M arrested single cells using nanoSPLITS
Single-cell proteomic data from RO-3306 treated alveolar type II epithelial cells (C10) and untreated C10 cells. Cells were sorted using cellenONE onto several nanoSPLITS chips. Cell lysates were then split for parallel RNAseq and proteomic analysis. For proteomics recovery experiment where both chi...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-12-02 | MSV000090828 | MassIVE
ProteomicsSingle-cell proteomics of Arabidopsis leaf mesophyll protoplasts in the context of drought stress
We followed the Arabidopsis tape-sandwich protocol to isolate the leaf protoplast enriched with palisade mesophyll cells. Briefly, control and WD-stressed Arabidopsis rosette leaves were placed on 3M tape, and abaxial cell layers were peeled off by gently removing the tape, thereby exposing the adax...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-06-18 | MSV000095072 | MassIVE
ProteomicsSpatially resolved top-down proteomics of tissue sections based on a microfluidic nanodroplet sample preparation platform
We demonstrate spatially resolved top-down proteomics (TDP) for proteoform identification and quantification from small sections of rat brain. We used the nanoPOTS (nanodroplet Processing in One pot for Trace Samples) platform, further improving the sample preparation by adding benzonase in the extr...
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) Homo Sapiens (ncbitaxon:9606) 
2022-03-30 | MSV000089163 | MassIVE
ProteomicsLarge scale, quantitative top-down proteomic analysis of human frontal gyrus brain tissue samples in the context of Alzheimer's disease
15-35 mg brain tissue plugs derived from dorsal lateral prefrontal cortex (DLPFC) were homogenized on ice with a pellet pestle homogenizer in a 1.5 mL LoBind Eppendorf tube after addition of 8 uL hexafluoroisopropanol (HFIP) / mg tissue. Once tissue was fully disrupted, an equivolume amount of homog...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-26 | MSV000093728 | MassIVE
ProteomicsDifferential solubilization and top-down proteomics with FAIMS control experiments applied to mouse brain
5-35 mg brain tissue plugs from mouse left or right hemisphere were homogenized on ice with a pellet pestle homogenizer in a 1.5 mL LoBind Eppendorf tube after addition of 8 uL hexafluoroisopropanol (HFIP) / mg tissue. Once tissue was fully disrupted, an equivolume amount of homogenization buffer (H...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-12-25 | MSV000093727 | MassIVE
ProteomicsMultimodal measurement of the transcriptome and proteome from dissociated human pancreatic islet cells using nanoSPLITS
Human pancreatic islets from two human donors (Prodo Laboratories) were acquired and cultured for 24 hr in pancreatic islet media (PIMS, Prodo Laboratories). Single cells were dissociated with TrypLE for ~15-30 minutes, washed with PBS, stained with Calcein-AM/propidium iodide, and sorted onto nanoS...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-07 | MSV000093330 | MassIVE
ProteomicsUltrasensitive Top-Down Proteomics Analysis of Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform
We developed an ultrasensitive top down platform by incorporating a microfluidic sample preparation system, termed nanoPOTS (Nanodroplet Processing in One pot for Trace Samples), into a top down workflow. We determined that a unique combination of a nonionic detergent DDM with urea as a protein extr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-22 | MSV000084612 | MassIVE
ProteomicsAn intelligent Boosting to Amplify Signal with Isobaric Labeling (iBASIL) strategy for precise quantitative single-cell proteomics
Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective proce...
ORGANISM(S): Homo Sapiens (human) 
2020-03-05 | PXD016057 | JPOST Repository
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