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ProteomicsProtein ‘Purity’, Proteoforms, and the Albuminome: Critical Observations on Proteome and Systems Complexity
The identification of effective, selective biomarkers and therapeutics is dependent on truly deep, comprehensive analysis of proteomes at the proteoform level. Using bovine serum albumin (BSA) isolated by two different protocols, cold ethanol fractionation (CEF) and heat shock fractionation (HSF), t...
ORGANISM(S): Bos taurus (Bovine) 
2024-12-07 | PXD056316 | Pride
Proteoforms Therapeutics Two-dimensional gel electrophoresis Tandem mass spectrometry Biomarkers Bovine serum albumin Integrative top-down proteomics 
ProteomicsLyophilization prior to homogenisation and extraction increases membrane protein detection in Gram-negative bacterial proteomic analyses
Multi-drug resistant Gram-negative bacteria (GNB) are major contributors to the anti-microbial resistance (AMR) burden in humans and animals. AMR mechanisms are primarily mediated by proteoforms and, therefore, proteomic analyses of GNB offers a significant advantage in understanding the mechanisms ...
ORGANISM(S): Escherichia coli 
2026-03-08 | PXD075198 | Pride
Proteomics Antimicrobial resistance Membrane proteins Bacterial protein extraction 
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