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Freshly collected abomasum tissue of the naturally infected sheep was ground, filtered, and centrifuged at 3000 g at 4 °C for cell collection. The collected cells were lysed in NP-40 lysis buffer (Thermo Fisher Scientific) for 0.5 h with shaking, then centrifuged at 12000 g at 4 °C for supernatant c...
ORGANISM(S): Ovis Aries 
MDA-MB-231 cells were irradiated with 10 Gy X-rays and the medium was harvest for extracellular vesicles isolation 24 hours post-irradiation. Proteins carrying by EVs of irradiated and control cells were then analysed by LC-MS.
ORGANISM(S): Homo Sapiens 
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