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ProteomicsRNA MS2-tag based affinity method was used to purify 3' processing factors associated with intronic PAS processing
three copies of the MS2 hairpin were fused to the intronic PAS of the nr3c1 gene. To make a negative control RNA substrate, the core hexamer AAUAAA within PAS was mutated to AACAAA. Subsequently, the RNA substrate was incubated with HeLa NE, and the mRNA 3' processing complex was purified using an M...
ORGANISM(S): Homo Sapiens 
2023-02-27 | PXD040405 |
ProteomicsProteomics data of extracellular vesicles, which are specifically derived from testicular macrophage cells within the mouse testis, offer detailed and comprehensive insights into the unique biochemical compositions and potential roles of these vesicles in the testicular microenvironment.
Testosterone synthesis in male mammals occurs predominantly in testicular Leydig cells (LCs), a process that is energetically demanding and results in cellular component damage. Despite this, LCs are long-lived and rarely renew, suggesting the presence of specialized mechanisms to maintain LCs homeo...
ORGANISM(S): Mus musculus (Mouse) 
2026-01-24 | PXD059652 | Pride
Testicular macrophage cells -evs 
ProteomicsProteomics data of extracellular vesicles, which are specifically derived from Leydig cells or testicular macrophage cells respectively within the mouse testis, offer detailed and comprehensive insights into the unique biochemical compositions and potential roles of these vesicles in the testicular microenvironment.
Testosterone synthesis in male mammals occurs predominantly in testicular Leydig cells (LCs), a process that is energetically demanding and results in cellular component damage. Despite this, LCs are long-lived and rarely renew, suggesting the presence of specialized mechanisms to maintain LCs homeo...
ORGANISM(S): Mus musculus (Mouse) 
2026-01-24 | PXD059780 | Pride
Leydig cells-evs 
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