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ProteomicsProtein accumulation post-LASIK surgery in granular corneal dystrophy type II patients involves altered proteolytic processing of transforming growth factor beta-induced protein (TGFBIp)
his study present the protein profile of diseased cornea from granular corneal dystrophy patients developing protein accumulation after LASIK surgery. Extracted ion chromatography (XIC) label free quantification was perform using Mascot Distiller software. In addition, 2D-PAGE followed by western bl...
ORGANISM(S): Homo sapiens (Human) 
2016-02-19 | PXD001997 | Pride
Cornea Tripletof 5600+ Xic quantification 2d-page Granular corneal dystrophy 
ProteomicsPeptide Filtering and Quantification Methods
In bottom-up proteomics, data are acquired on peptides resulting from proteolysis. In XIC-based quantification, the quality of the protein abundance estimation depends on how peptide data are filtered and on which quantification method is used to sum up peptide intensities into protein abundances. S...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-11-14 | PXD009740 | Pride
Peptide filtering Quantification methods Extracted ion current 
ProteomicsAn XIC-centric approach for improved identification and quantification in proteomic data analyses
An XIC-centric approach for improved identification and quantification in proteomic data analyses
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-01-16 | MSV000093875 | MassIVE
ProteomicsProteomics of Fuchs Endothelial Corneal Dystrophy support that the extracellular matrix of Descemet¹s membrane is Disordered
Fuchs’ endothelial corneal dystrophy is major corneal disorder in the western world affecting the innermost part of the cornea, which leads to visual impairment. The morphological changes observed in Fuchs’ endothelial corneal dystrophy is well described, however, much less in known of the pathology...
ORGANISM(S): Homo sapiens (Human) 
2014-05-27 | PXD000746 | Pride
Itraq quantification Fuchs' endothelial corneal dystrophy Xic quantification 
ProteomicsMedian Based Absolute Quantification using Fully Unlabelled Generic Internal Standard
By reporting molar abundances of proteins, absolute quantification determines their stoichiometry in complexes, pathways or networks and also relates them to abundances of non-protein biomolecules. Typically, absolute quantification relies either on protein- specific isotopically labelled peptide st...
ORGANISM(S): Homo sapiens (Human) Caenorhabditis elegans 
2021-11-30 | PXD026886 | Pride
Absolute quantification 
ProteomicsHost Cell Protein Quantification Workflow Using Optimized Standards combined with Data-Independent Acquisition Mass Spectrometry
Monitoring of host cell proteins (HCPs) during the manufacture of monoclonal antibodies (mAb) has become a critical requirement to provide effective and safe drug product. ELISA assays are still the current gold standard for the quantification of protein impurities. However, this technique has sever...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-07-20 | PXD029305 | Pride
Hcp profiler Protein quantification Data validation. Data-independent acquisition (dia) Host cell proteins (hcps) 
UnknownComparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance.
Not available
S-EPMC8788469 | biostudies-literature
ProteomicsReproducibility, specificity and accuracy of DIA quantification - DIA analysis
For the complete description see the manuscript. Data dependent acquisition (DDA) is the method of choice for mass spectrometry based proteomics discovery experiments, data-independent acquisition (DIA) is steadily becoming more important. One of the most important requirement to perform a DIA analy...
ORGANISM(S): Mus musculus (Mouse) 
2019-11-08 | PXD012988 | Pride
Dia Label-free quantification Spectral library Data-independent acquisition 
MetabolomicsQuantitative profiling of endocannabinoids in lipoproteins by LC–MS/MS
Endocannabinoids belong to a diverse family of endogenous lipid bioregulators acting as physiological ligands of cannabinoid receptor type 1 and cannabinoid receptor type 2 in the central and peripheral nervous system. They are also present in nmol/l concentrations in human blood plasma; however, th...
2015-03-11 | MTBLS163 | MetaboLights
ProteomicsQuantification of amyloid protein enrichment by mass spectrometry improves typing of amyloidosis
Amyloidosis typing is crucial to determine the best therapeutic strategy for patients. Since conventional histological techniques often fail, the identification of amyloid precursors by mass spectrometry became the new standard. However, without quantification, selecting the amyloid precursor from p...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD039814 | Pride
Proteomics Mass spectrometry Label-free quantification Amyloidosis typing 
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