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A sulfur-containing analog of tryptophan, thiotryptophan (3-benzothienyl-L-alanine, S-Trp), was genetically incorporated into EcKatG to replace the MYW-forming tryptophan, W105. The S-Trp-bearing peptide was isolated using trypsin digestion followed by HPLC separation. Tandem mass spectrometry (MS/M...
ORGANISM(S): Escherichia coli 
2026-05-13 | PXD078006 | Pride
A sulfur-containing analog of tryptophan, thiotryptophan (3-benzothienyl-L-alanine, S-Trp), was genetically incorporated into EcKatG to replace the MYW-forming tryptophan, W105. The heme free apo-protein was purified, after heme-reconstitution and peroxide treatment, the S-Trp-bearing peptide was is...
ORGANISM(S): Escherichia coli 
2026-05-13 | PXD078069 | Pride
Natural killer T (NKT) cells are important therapeutic targets in various disease models and under clinical trials for cancer patients. However, their function in obesity and type 2 diabetes remains unclear. Our data show that adipose tissues of both mice and humans contain a population of type-1 NK...
ORGANISM(S): Mus musculus 
A growing body of evidence suggests interplay between the gut microbiota and the pathogenesis of nonalcoholic fatty liver disease (NAFLD). However, the role of the gut microbiome in early detection of NAFLD is unclear. Prospective studies are necessary for identifying reliable, microbiome markers fo...
2022-07-11 | MTBLS2615 | MetaboLights
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