The promiscuous biotin ligase TurboID was fused to Tat for precise and temporal biotinylation of surrounding proteins, which could be enriched by streptavidin and identified by mass spectrometry.
This project identifies GALNT1-interacting proteins in primary fibroblast-like synoviocytes (RASFs) derived from rheumatoid arthritis patients using co-immunoprecipitation (co-IP) coupled with mass spectrometry (MS). Briefly, RASF lysates were incubated with anti-GALNT1 antibody to capture GALNT1-a...
