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ProteomicsStress Granule-associated ZmCTU2 Confers Thermotolerance in Maize via Coordinated Regulation of Proteostasis and ROS Homeostasis
we performed comparative proteomic analysis of wild-type B104 and ZmCTU2-overexpressing lines. Seedlings at three-leaf-stage were grown under normal temperature condition (NC; 25°C for 12 days) and subsequently subjected to heat stress (HS; 43°C for 8 hours following NC growth). Using tandem mass ta...
ORGANISM(S): Zea Mays 
2025-12-17 | PXD072171 |
ProteomicsBRD4 regulates the osteogenic differentiation of MSCs
MSCs were lysed in cell lysis buffer for Western blotting and IP (Beyotime, Cat. No. P0013). A Dynabeads™ protein G immunoprecipitation kit (Invitrogen, Cat. No. 10007D) was used for Co-IP according to the manufacturer’s instructions. Dynabeads were resuspended and placed on a magnet to remove the s...
ORGANISM(S): Homo Sapiens 
2022-06-16 | PXD034615 |
ProteomicsMethylcobalamin protects against liver failure via engaging gasdermin E
To probe the binding sites of MeCbl on GSDME, 0.4 μM recombinant human GSDME (CSB-EP006766HU) purchased from CUSABIO (Wuhan, China) was incubated with 10 μM MeCbl in 100 μL buffer (20 mM HEPES, 150 mM NaCl and 50 μM DPA) at room temperature for 2 h and protected from light. 0.4 μM recombinant human ...
ORGANISM(S): Homo Sapiens 
2024-11-11 | PXD057768 |
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