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Proteomics[HPP-2.0] Phosphoproteome Profiling of uEV Reveals p-AQP2 and p-GSK3β as Potential Markers for Diabetic Nephropathy-2
We used ultra-high-sensitivity quantitative phosphoproteomics to identify phosphoproteins in urinary extracellular vesicles (uEVs) as potential biomarkers of DN. We detected 233 phosphopeptides within the uEVs, with 47 phosphoproteins exhibiting significant alterations in patients with DN compared t...
ORGANISM(S): Homo Sapiens Bos Taurus 
2024-02-20 | PXD049714 |
Proteomics[HPP-2.0] Phosphoproteome Profiling of uEV Reveals p-AQP2 and p-GSK3β as Potential Markers for Diabetic Nephropathy
We used ultra-high-sensitivity quantitative phosphoproteomics to identify phosphoproteins in urinary extracellular vesicles (uEVs) as potential biomarkers of DN. We detected 233 phosphopeptides within the uEVs, with 47 phosphoproteins exhibiting significant alterations in patients with DN compared t...
ORGANISM(S): Homo Sapiens 
2024-02-01 | PXD049107 |
ProteomicsMass spectrometry of HCT116 cells
HCT116 cells were transfected with flag-pcDNA3.1 plasmids or flag-MST3 plasmids. Whole-cell extracts were prepared with a lysis buffer, lysed on ice for 10 min, centrifuged at 12,000 rpm for 10 min at 4°C, and the supernatant was collected. The anti-Flag antibody and protein A/G beads were added and...
ORGANISM(S): Homo Sapiens 
2024-03-09 | PXD050513 |
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