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UnknownTrivalent Phospha-Brook rearrangement enabled practical deoxygenative phosphonylation of carbonyls.
Brønsted base-mediated [1,2]-phospha-Brook rearrangements have garnered considerable attention for developing new methodologies and efficiently constructing complex molecular structures. However, the strict reliance on pentavalent phosphonates imposes strong limitations on the reaction types, and th...
S-EPMC12003827 | biostudies-literature
UnknownRhizosphere protists are key determinants of plant health.

Background

Plant health is intimately influenced by the rhizosphere microbiome, a complex assembly of organisms that changes markedly across plant growth. However, most rhizosphere microbiome research has focused on fractions of this microbiome, particularly bacteria and fungi. It remains un...
S-EPMC7055055 | biostudies-literature
UnknownExploring rhizo-microbiome transplants as a tool for protective plant-microbiome manipulation.
The development of strategies for effectively manipulating and engineering beneficial plant-associated microbiomes is a major challenge in microbial ecology. In this sense, the efficacy and potential implications of rhizosphere microbiome transplant (RMT) in plant disease management have only scarce...
S-EPMC9723603 | biostudies-literature
UnknownLorBin: efficient binning of long-read metagenomes by multiscale adaptive clustering and evaluation.
Long-read sequencing has transformed metagenomics and improved the quality of metagenome-assembled genomes (MAGs). However, current binning methods struggle with identifying unknown species and managing imbalanced species distributions. Here, we present LorBin, an unsupervised binner specially desig...
S-EPMC12552423 | biostudies-literature
UnknownA new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris.

Background

Endo-1,4-β-mannanase is an enzyme that can catalyze the random hydrolysis of β-1, 4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans and has a number of applications in different biotechnology industries. Penicillium oxalicum is a powerful hemicell...
S-EPMC4219100 | biostudies-literature
ProteomicsThe phosphorylation sites in GLI1 analyzed by mass-spec methodology
The mass spectrometry analysis was performed by Micrometer Biotech Company. Endogenous human GLI1 from Daoy cells was enriched by co-immunoprecipitation with anti-Gli1 and purified by SDS-PAGE. The gel was then stained with Coomassie brilliant blue, and the corresponding visualized bands were excise...
ORGANISM(S): Homo Sapiens 
PXD046374 |
UnknownEffects of plant tissue permeability on invasion and population bottlenecks of a phytopathogen.
Pathogen genetic diversity varies in response to environmental changes. However, it remains unclear whether plant barriers to invasion could be considered a genetic bottleneck for phytopathogen populations. Here, we implement a barcoding approach to generate a pool of 90 isogenic and individually ba...
S-EPMC10762237 | biostudies-literature
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