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TranscriptomicsA large-scale clinical study of gene expression response to severe burn injury
To understand the age-dependent response to burn injury, blood samples from pediatric and adult patients were collected at different times after severe burn injury. Gene expression was measured using Affymetrix U133 Plus 2.0 arrays for both patient samples and healthy controls. Time points were binn...
ORGANISM(S): Homo sapiens 
2010-06-16 | E-GEOD-19743 | biostudies-arrayexpress
TranscriptomicsA human transcriptome array for high-throughput clinical studies (with a comparison to RNA-seq technology) [RNA-Seq]
A new high-density oligonucleotide array of the human transcriptome (GG-H array) has been developed for high-throughput and cost-effective analyses in clinical studies. This array allows comprehensive examination of gene expression and genome-wide identification of alternative splicing, as well as ...
ORGANISM(S): Homo sapiens 
2011-01-27 | E-GEOD-26109 | biostudies-arrayexpress
TranscriptomicsIdentification of quantitative trait loci controlling gene expression during the innate immunity response of soybean
Microbe associated molecular pattern (MAMP)-responsive genes were identified in leaves of four different genotypes. The genotypes used in this study were: LD0-2817P; LDX01-1-65; Ripley and EF59. Leaves from three-weeks old plants were used in this study We used microarray to identified the MAMP-resp...
ORGANISM(S): Glycine max 
2011-10-05 | E-GEOD-32642 | biostudies-arrayexpress
TranscriptomicsA human transcriptome array for high-throughput clinical studies (with a comparison to RNA-seq technology) [expression array]
A new high-density oligonucleotide array of the human transcriptome (GG-H array) has been developed for high-throughput and cost-effective analyses in clinical studies. This array allows comprehensive examination of gene expression and genome-wide identification of alternative splicing, as well as ...
ORGANISM(S): Homo sapiens 
2011-01-27 | E-GEOD-26072 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of neutrophils isolated by microfluidics: Clinical Microfluidics for Neutrophil Genomics and Proteomics
Neutrophils play critical roles in modulating the immune response. However, neutrophils have a short circulating half life, are readily stimulated in vitro, and have low levels of cellular mRNA when compared to other blood leukocyte populations. All of these factors have made it difficult to evaluat...
ORGANISM(S): Homo sapiens 
2010-06-16 | E-GEOD-22103 | biostudies-arrayexpress
TranscriptomicsProfiles of gene expression in skeletal muscle in sheep from the middle gestation to postnatal stages
Texel and Ujumqin sheep show obvious differences in muscle and fat growth, so they are ideal models not only to understand the molecular mechanism in prenatal skeletal muscle development, but to identify the potential target genes of myostatin. To elucidate the phenotypic variation between the two s...
ORGANISM(S): Ovis aries 
2010-12-20 | E-GEOD-23563 | biostudies-arrayexpress
TranscriptomicsA comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequence Quality Control consortium
We present primary results from the Sequencing Quality Control (SEQC) project, coordinated by the United States Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites using reference RNA samples with built-in controls, we ...
ORGANISM(S): Homo sapiens 
2014-08-08 | E-GEOD-56457 | biostudies-arrayexpress
TranscriptomicsA genomic storm in critically injured humans
Human survival from injury requires an appropriate inflammatory and immune response. We describe the circulating leukocyte transcriptome after severe trauma and show that the severe stress produce a global reprioritization affecting >80% of the cellular functions and pathways, a truly unexpected “ge...
ORGANISM(S): Homo sapiens 
2012-03-26 | E-GEOD-36809 | biostudies-arrayexpress
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