Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

A genomic storm in critically injured humans

ABSTRACT: Human survival from injury requires an appropriate inflammatory and immune response. We describe the circulating leukocyte transcriptome after severe trauma and show that the severe stress produce a global reprioritization affecting >80% of the cellular functions and pathways, a truly unexpected “genomic storm.” In severe blunt trauma, the early leukocyte genomic response is consistent with simultaneously increased expression of genes involved in the systemic inflammatory, innate immune, and compensatory antiinflammatory responses, as well as in the suppression of genes involved in adaptive immunity. Furthermore, complications like nosocomial infections and organ failure are not associated with any genomic evidence of a second hit and differ only in the magnitude and duration of this genomic reprioritization. Blood was sampled from 167 severe blunt trauma patients under the age of 55 yr who consented to blood sampling. The first blood sample was taken within 12 h of the injury and 1, 4, 7, 14, 21, and 28 d after injury. Study subjects were treated under the guidance of standard operating procedures developed, implemented, and audited across all participating centers to minimize treatment variation. In addition, 37 healthy control subjects were sampled. Total blood leukocytes were isolated according to protocols published previously. Total cellular RNA was extracted and hybridized onto an HU133 Plus 2.0 GeneChip (Affymetrix) according to the manufacturer’s recommendations.

ORGANISM(S): Homo sapiens

SUBMITTER: IDDC Inflammation & the Host Response to Injury

PROVIDER: E-GEOD-36809 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A genomic storm in critically injured humans.

Xiao Wenzhong W Mindrinos Michael N MN Seok Junhee J Cuschieri Joseph J Cuenca Alex G AG Gao Hong H Hayden Douglas L DL Hennessy Laura L Moore Ernest E EE Minei Joseph P JP Bankey Paul E PE Johnson Jeffrey L JL Sperry Jason J Nathens Avery B AB Billiar Timothy R TR West Michael A MA Brownstein Bernard H BH Mason Philip H PH Baker Henry V HV Finnerty Celeste C CC Jeschke Marc G MG López M Cecilia MC Klein Matthew B MB Gamelli Richard L RL Gibran Nicole S NS Arnoldo Brett B Xu Weihong W Zhang Yuping Y Calvano Steven E SE McDonald-Smith Grace P GP Schoenfeld David A DA Storey John D JD Cobb J Perren JP Warren H Shaw HS Moldawer Lyle L LL Herndon David N DN Lowry Stephen F SF Maier Ronald V RV Davis Ronald W RW Tompkins Ronald G RG

The Journal of experimental medicine 20111121 13

Human survival from injury requires an appropriate inflammatory and immune response. We describe the circulating leukocyte transcriptome after severe trauma and burn injury, as well as in healthy subjects receiving low-dose bacterial endotoxin, and show that these severe stresses produce a global reprioritization affecting >80% of the cellular functions and pathways, a truly unexpected "genomic storm." In severe blunt trauma, the early leukocyte genomic response is consistent with simultaneously ...[more]

PMID: 22110166

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