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MetabolomicsSingle-Cell and Spatial Multi-Omics Reveal Mechanical Stress Driving Heterogeneous Xylem Development in Populus [Spatial metabolomics_LCM]
Xylem, the predominant tissue for structural support, forms tension wood with G-layer-rich fibers under mechanical stress. Despite being recognized over a century ago, three key biological questions remained unclear: (1) Are fibers in normal and tension wood distinct cells due to morphological diffe...
2025-06-16 | MTBLS12561 | MetaboLights
ProteomicsActive protein ubiquitination regulates xylem vessel functionality
Xylem vessels function in the long-distance conduction of water in land plants. The NAC transcription factor VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a master regulator of xylem vessel cell differentiation in Arabidopsis (Arabidopsis thaliana). We previously isolated seiv (suppressor of ectopic xylem ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-10-17 | PXD048830 | Pride
Vnd7 Xylem vessel Ubiquitination 
TranscriptomicsTranscription profiling of Arabidopsis wild type, MYB46 over-expressing, MYB83 over-expressing and myb46/myb83 double mutants to investigate the regulation mechanism of secondary cell wall synthesis during xylem vessel differentiation
To investigate the regulation mechanism of secondary cell wall synthesis during xylem vessel differentiation, we analyzed the function of two Arabidopsis MYB transcription factors, MYB46 and MYB83. Microarray analysis was performed for 11-day old seedlings of wild-type, MYB46 over-expressor, MYB83 o...
ORGANISM(S): Arabidopsis thaliana 
2010-05-01 | E-MEXP-2445 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis in vitro tracheary element transdifferentiation of Col-0 suspension cells.
This experiment was annotated by TAIR (http://arabidopsis.org). Col-0 suspension cells provided by Dr. C. Koncz and Dr. M. Umeda. 15 ml of Arabidopsis Col-0 suspension cells (Mathur et al. 1998) was transferred to 35 ml of a fresh modified Murashige and Skoog (MS) medium supplemented with 1 ug /ml 2...
ORGANISM(S): Arabidopsis thaliana 
2008-06-14 | E-GEOD-5748 | biostudies-arrayexpress
TranscriptomicsFinding direct target genes of VND7
Direct target genes of VND7 were explored with inducible expression system using glucocorticoid receptor (GR). Transgenic plants expressing 35S:VND7-VP16-GR were treated with dexamethazone (DEX) and/or protein synthesis inhibitor cycloheximide (CHX). A number of genes related to the formation of vas...
ORGANISM(S): Arabidopsis thaliana 
2011-03-31 | E-GEOD-24169 | biostudies-arrayexpress
ProteomicsNUT1-Exo70A1 Regulates Xylem Vessel Development and Influences Water Use Efficiency in Maize
The total proteins from transgenic plants overexpressing Exo70A1 coding sequence in a translational fusion of a GFP-tag were extracted from root stele tissues using a lysis buffer composed of 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5 mM EDTA pH 8.0, 0.5% NP-40, and freshly added 1 mM PMSF, 1 mM DTT, 1...
ORGANISM(S): Zea Mays 
2026-01-20 | PXD073273 |
UnknownActive protein ubiquitination regulates xylem vessel functionality.
Not available
S-EPMC11371170 | biostudies-literature
UnknownXylem-dwelling pathogen unaffected by local xylem vessel network properties in grapevines (Vitis spp.).
Not available
S-EPMC11037485 | biostudies-literature
TranscriptomicsIsolation of poplar genes involved in xylem vessel differentiation
Downstream genes of PtVNS genes were explored with inducible expression system using glucocorticoid receptor (GR). Transgenic poplar plants expressing 35S:AtVND7-VP16-GR were treated with dexamethazone (DEX). A number of genes related to the formation of xylem vessels were induced by DEX. Total RNAs...
ORGANISM(S): Populus tremula x Populus tremuloides 
2011-04-30 | E-GEOD-25304 | biostudies-arrayexpress
UnknownSalicylic acid-mediated competitive regulation of xylem vessel formation and defense responses in Arabidopsis thaliana.
Not available
S-EPMC12461850 | biostudies-literature
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