Dataset Information

NUT1-Exo70A1 Regulates Xylem Vessel Development and Influences Water Use Efficiency in Maize

ABSTRACT: The total proteins from transgenic plants overexpressing Exo70A1 coding sequence in a translational fusion of a GFP-tag were extracted from root stele tissues using a lysis buffer composed of 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5 mM EDTA pH 8.0, 0.5% NP-40, and freshly added 1 mM PMSF, 1 mM DTT, 1× protease inhibitor cocktail. After incubation for 40 min at 4 °C, the lysates were centrifuged twice at 13,400 g for 10 min at 4 °C. The cleared protein extracts were then subjected to immunoprecipitation using an GFP Nanoab Agarose beads (NuoyiBio, Cat#AGA-50-1K). The mixture was incubated for 3 h at 4 °C. Subsequently, the precipitated samples were washed 3 times with a wash buffer containing 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, pH 8.0, 0.5% NP-40. Finally, the precipitated samples were washed 3 times with 1× PBS (EASYBIO, Cat#BE6258). The resulting protein samples were analyzed by western blotting using an anti-GFP (ABclonal, Cat#AE012) and further processed for LC-MS/MS analysis.

ORGANISM(S): Zea Mays

SUBMITTER: Feng Qin

PROVIDER: PXD073273 | iProX | Tue Jan 20 00:00:00 GMT 2026

REPOSITORIES: iProX

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NUT1-Exo70A1 Regulates Xylem Vessel Development and Influences Water Use Efficiency in Maize.

Zhu Tengfei T Wang Yanyan Y Wang Yijie Y Wang Chen C Liu Yu Y He Jiafan J Zhao Bochen B Liu Shengxue S Lian Yongyan Y Wu Liuji L Cheng Jinkui J Yang Zhirui Z Qin Feng F

Nature communications 20260216 1

Efficient water uptake and transport through xylem vessels are essential for plant growth and development. The patterned secondary cell wall (SCW) structure of xylem vessels provides robust mechanical support to withstand the strong negative pressure generated by transpiration and facilitates long-distance water transport. However, the key factors governing SCW patterning in xylem vessels and their potential for enhancing water use efficiency (WUE) remain undetermined. Here, we report the identi ...[more]

PMID: 41698926

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Dataset Information

NUT1-Exo70A1 Regulates Xylem Vessel Development and Influences Water Use Efficiency in Maize

Publications

NUT1-Exo70A1 Regulates Xylem Vessel Development and Influences Water Use Efficiency in Maize.

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