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∆MIC11::MIC11-Spot-HA parasites or the parental ∆MIC11 parasites were fixed with 0.1% PFA and lysed in HEPES-RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 1 mM EGTA, 1 mM MgCl2, 150 mM NaCl, 0.25% sodium deoxycholate, 0.05% SDS, 1% NP-40) supplemented with protease inhibitor cocktail cOmplete EDTA-free (Ro...
ORGANISM(S): Cellular Organisms 
Control (ROP9-3HA) parasites or PLP1-3HA expressing parasites were fixed with 0.1% PFA and lysed in HEPES-RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 1 mM EGTA, 1 mM MgCI2, 150 mM NaCl, 0.25% sodium deoxycholate, 0.05% SDS, 1% NP-40) supplemented with protease inhibitor cocktail cOmplete EDTA-free (Roche...
ORGANISM(S): Cellular Organisms 
To extract the molecules that correlate with kidney function and fibrosis, we performed the proteome analysis of non-cancer lesion of renal cell carcinoma in 16 patients. Proteins were extracted from FFPE slice samples and digested using the SP3 method as previously described. The digested peptide s...
ORGANISM(S): Homo Sapiens (human) 
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