Parental and PKD2/3 double-deficient DN32.D3 cells were treated with 10 ug/ml anti-CD3 and cross-linked with 100 ug/ml goat anti-Hamster IgG for 5 min. Cells were washed with ice-cold HEPES-saline and lysed with Guanidine-HCl buffer (6 M guanidine-HCl, 100 mM Tris-Cl pH8.0, 2 mM DTT). The lysates we...
ORGANISM(S): Mus Musculus (mouse)
