Huh7 cells with expression of Flag-GPX4 were treated with IGF1. An immunoprecipitation assay was performed, and immunoprecipitates were subjected to masspec analysis.
4D label-free quantitative lactylproteomics analysis was performed to identify the differentially lactylated proteins between human normal mammary epithelial cell line (MCF 10A) and BC cells (MCF7, T-47D, and MDA-MB-468)