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TranscriptomicsRetionic acid gene expression time series in human embryonic stem cells
Retinoic acid RA time course experiment: H1 cells(WiCell Research Institute, WA01, Passage40) maintained in TeSR1 were treated with 1uM retinoic acid (RA) in a detailed time course:day0,day0.5,day1,day1.5,day2,day3, day4 and day5 . Total RNA was prepared by usage of the Qiagen RNA extraction kit. m...
ORGANISM(S): Homo sapiens 
2007-09-13 | E-GEOD-8439 | biostudies-arrayexpress
TranscriptomicsWhole genome ChIP/chip tiling of human ES cells for H3K4me3 and H3K27me3
Chromatin immunoprecipitation was performed using monoclonal antibodiesd. DNA was amplified, fluorescently labeled and hybridized to a series of 38 arrays containing a total of 14.6 million 50-mer oligonucleotides Keywords: ChIP-chip hES whole genome Chromatin immunoprecipitated DNA from H3K4me
ORGANISM(S): Homo sapiens 
2007-09-14 | E-GEOD-8463 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse HNF4 null mutant colon vs. control colons
Background and Aims: HNF4? is a nuclear hormone receptor transcription factor that has been shown to be required for hepatocyte differentiation and development of the liver. It has also been implicated in regulating expression of genes that act in the epithelium of the lower gastrointestinal tract....
ORGANISM(S): Mus musculus 
2008-06-12 | E-GEOD-3116 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wild type mouse livers and livers from hepatocyte nuclear factor 4alpha deficient mice to study the role of HNF4a in hepatogenesis.
To study the role of hepatic nuclear factor _ alpha (HNF4a_ in hepatogenesis, we used loxP-Cre technology to eliminate it from developing mouse livers. A comparison of control and experimental livers revealed that hepatocytes lacking HNF4a_failed to fully differentiate. Specifically, HNF4a null l...
ORGANISM(S): Mus musculus 
2007-12-01 | E-GEOD-3126 | biostudies-arrayexpress
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