Sort   by:  
 Page size 
TranscriptomicsRNA-biotin based pull down assay with polyA site (PAS) RNA substrates in Hela Nuclear Extract followed by RNA-seq to discover trans-acting RNA molecules involved in mRNA 3 processing
We aimed to discover trans-acting RNA molecules involved in mRNA 3 processing. We reasoned that, if there exist such functional RNAs, they must directly associate with the key machinery responsible for mRNA 3 processing. Therefore, it would be of great value to comprehensively identify RNAs interact...
ORGANISM(S): Homo sapiens 
2017-06-01 | E-MTAB-5383 | biostudies-arrayexpress
TranscriptomicspolyA site sequencing (PAS-seq) analysis of Hela cells treated with NC ASO or snoRD50a ASOs
Our RNA-seq experiment (E-MTAB-5383) and subsequent in vitro analysis suggested that snoRD50a is a trans-acting RNA that can function in mRNA 3' processing. To test this hypothesis at transcriptomic level, we performed PAS-seq experiments to compare the polyA+ transcripts profile between negative co...
ORGANISM(S): Homo sapiens 
2017-05-31 | E-MTAB-5389 | biostudies-arrayexpress
ProteomicsRNA MS2-tag based affinity method was used to purify 3' processing factors associated with intronic PAS processing
three copies of the MS2 hairpin were fused to the intronic PAS of the nr3c1 gene. To make a negative control RNA substrate, the core hexamer AAUAAA within PAS was mutated to AACAAA. Subsequently, the RNA substrate was incubated with HeLa NE, and the mRNA 3' processing complex was purified using an M...
ORGANISM(S): Homo Sapiens 
2023-02-27 | PXD040405 |
TranscriptomicsCoupling between alternative polyadenylation and alternative splicing is limited to terminal introns
Alternative polyadenylation has been implicated as an important regulator of gene expression. In some cases, alternative polyadenylation is known to couple with alternative splicing to influence last intron removal. However, it is unknown whether alternative polyadenylation events influence alternat...
ORGANISM(S): Homo sapiens 
2016-03-14 | E-GEOD-79157 | biostudies-arrayexpress
Sort   by:  
 Page size