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Attachment of the ubiquitin (UB) peptide to proteins via the E1-E2-E3 enzymatic machinery regulates diverse biological pathways, yet identification of the substrates of E3 UB ligases remains a challenge. We overcame this challenge by constructing an “orthogonal UB transfer (OUT) cascade with yeast E...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-13 | PXD023688 | Pride
Microarrays were used to identify Y. pestis genes that are transcriptional targets of phoP. RNA samples were isolated from cultures of two isogenic Y. pestis strains, one carrying wild-type phoP (KIM6+) and the other carrying a deletion within phoP (KIM6+phoP(delta)), and their gene expression prof...
ORGANISM(S): Yersinia pestis 
Microarrays were used to identify Y. pestis genes that are differentially regulated under conditions of phoP overexpression. RNA samples were isolated from cultures of two isogenic Y. pestis strains, one overexpressing phoP (KIM10+phoP(delta)/PhoP) and the other not (KIM10+phoP(delta)/MMB67EH), and ...
ORGANISM(S): Yersinia pestis 
We investigated the differential expression of the total membrane proteins of the two sperm types by using high-purity X- and Y-sperm from 20 Holstein bulls and applying the label-free proteomic technique.
ORGANISM(S): Bos Taurus 
The study tried the identify some differentially expressed proteins in rat lingual keratinocytes treated with or without the ROCK inhibitor Y-27632. In brief, rat lingual keratinocytes were treated with or without Y-27632 for 36 h and were subjected to proteomic analysis.
ORGANISM(S): Cellular Organisms 
We generated skeletal muscle-specific knockout mice lacking the transcription factor Yin Yang 1 (YY1) and analyzed expression patterns in the skeletal muscle these mice. We used microarrays to detail the global programme of gene expression regulated by YY1. Wild type or knockout mice at 6 months wer...
ORGANISM(S): Mus musculus 
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