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The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-so...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-20 | MSV000080256 | MassIVE
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-so...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-20 | MSV000080254 | MassIVE
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-s...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-20 | MSV000080255 | MassIVE
Small interfering RNAs (siRNAs) are the key components for RNA interference (RNAi), a conserved RNA silencing mechanism in many eukaryotes. In Drosophila, an RNase III enzyme Dicer-2 (Dcr-2), aided by its cofactor Loquacious-PD (Loqs-PD), plays major role in generating 21 base-pair (bp) siRNA duplex...
ORGANISM(S): Drosophila Melanogaster 
2022-04-21 | PXD033365 |
CHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, part 1
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005189 | PeptideAtlas
CHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, part 2
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005197 | PeptideAtlas
CHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, part 3
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005194 | PeptideAtlas
The protein samples stored at -80 °C were thawed and treated with lysis buffer (8M urea, 1% protease inhibitor, 50mM NH4HCO3) for 5 minutes in room temperature. After ultrasonic cracking on ice and centrifugation at 14000g for 10 min at 20 °C, the amount of total protein of each MSC sample was detec...
ORGANISM(S): Homo Sapiens 
2023-06-12 | PXD042977 |
The protein samples stored at -80 °C were thawed and treated with lysis buffer (8M urea, 1% protease inhibitor, 50mM NH4HCO3) for 5 minutes in room temperature. After ultrasonic cracking on ice and centrifugation at 14000g for 10 min at 20 °C, the amount of total protein of each MSC sample was detec...
ORGANISM(S): Homo Sapiens 
2023-06-12 | PXD033812 |
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