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TranscriptomicsMouse cytokine antibody array
Tumors of hematopoietic stem cell transplantation (HSCT) or control mice were collected at day 14 post transplantation. Comprehensive analysis of cytokine levels were performed by using commercially available RayBio Mouse Cytokine Array G series III and IV (RayBiotech, Inc.) according to manufacture...
ORGANISM(S): Mus musculus 
2013-08-28 | E-MTAB-1761 | biostudies-arrayexpress
ProteomicsPrimPol-phosphoproteomics by IP-GeLC-MS/MS
Myc-tagged PrimPol expressed in HEK-293T cells was immunoprecipitated and separated by SDS-PAGE. Protein bands were excised and subjected to in-gel tryptic digestion and phosphopeptide enrichment by Fe-IMAC.
ORGANISM(S): Homo Sapiens (human) 
2023-08-22 | PXD043419 | JPOST Repository
ProteomicsTMTpro-18plex analysis of MM1.S cells treated with thalidomide and its derivatives
MM1.S cells were treated with DMSO or 10 µM thalidomide or its derivatives for 5 h in two biological replicates. The cells were lysed in 200 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonication, proteins (100 µg each) wer...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD041812 | JPOST Repository
ProteomicsTMTpro-18plex analysis of NTERA-2 cells treated with PROTACs
NTERA-2 cells were treated with DMSO, 0.3 µM Po-P, 0.3 µM Le-P, 0.3 µM F-P, 0.3 µM Cl-P, or 0.3 µM F3C-P for 16 h in three biological replicates. The cells were lysed in 100 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonic...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD037179 | JPOST Repository
ProteomicsTMTpro-18plex analysis of MM1.S cells treated with PROTACs
MM1.S cells were treated with DMSO, 0.3 µM Po-P, 0.3 µM Le-P, 0.3 µM F-P, 0.3 µM Cl-P, or 0.3 µM F3C-P for 16 h in three biological replicates. The cells were lysed in 100 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonicat...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD037178 | JPOST Repository
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