Here we employed a non-enzymatic chemical labeling approach to introduce lactylation on lysine residues of model peptides and proteins. Tandem MS acquisition of lactylated peptide carriers on different MS platforms unequivocally led us to note an intense peak corresponding to the “cyclic” immonium ...
We established deep-coverage thyroid-specific spectral libraries encompassing over 12,000 proteins, including samples from nine types of thyroid primary and lymph node metastases tissues and fifty thyroid cancer cell lines.
