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ProteomicsProteome profiling of Cumin by Q-Exactive HF mass spectrometer
A shotgun proteomic approach was used to obtain comprehensive dataset of cumin proteins. For this purpose we applied a one dimensional electrophoresis coupled with high resolution mass spectrometry. The first step in our proteome analysis strategy, we evaluate different extraction methods, based on ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-11-21 | PXD006257 | Pride
Plant proteins Lcms 
ProteomicsIn depth proteome profiling of Cumin by LTQ-Orbitrap Velos mass spectrometer
A shotgun proteomic approach was used to obtain comprehensive dataset of cumin proteins. For this purpose we applied a one dimensional electrophoresis coupled with high resolution mass spectrometry. The first step in our proteome analysis strategy, we evaluate different extraction methods, based on ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-28 | PXD006425 | Pride
Plant proteins Cumin Lc-ms/ms 
ProteomicsDithiothreitol (DTT) acts as a specific, UV-inducible cross-linker in elucidation of protein-RNA interactions
In the present paper, we introduce dithiothreitol (DTT) as a potent protein-RNA cross-linker. To prove this three model systems, a) a small synthetic peptide from smB'/B protein incubated with U1snRNA oligonucleotide, b) in vitro reconstituted 15.5K protein with U4 snRNA oligonucleotide and c) nativ...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2015-10-13 | PXD002656 | Pride
Dtt 
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