Manduca sexta is a lepidopteran model widely used to study insect physiological processes including innate immunity. In this study, we explored the proteomes of cell-free hemolymph from larvae injected with a sterile buffer (C for control) or a mixture of bacteria (I for induced). Of the 654 protein...
The promiscuous biotin ligase TurboID was fused to Tat for precise and temporal biotinylation of surrounding proteins, which could be enriched by streptavidin and identified by mass spectrometry.
To examine whether P26 is involved in the extracellular melanization pathway, we used HearNPV and cotton bollworm as a testing system and detected the presence of HearNPV P26 in hemolymph from the virus-infected insect via liquid chromatography tandem mass spectrometry analysis. P26 was one of the m...
