TGM2 IP-MS was performed in MHCC-97H cells to screen the interactors of TGM2. 5-PT pulldown-MS was perfromed in MHCC-97H cells to identify proteins modified by serotonylation.
In brief, U251 cells lysate were incubated with 10μM KpKla and 10μM KpKla separately. After incubating for 1h at 37℃, the solutions were irradiated by 365nm UV for 5 min on ice. Then CuSO4, THPTA (tris-hydroxypropyltriazolylmethylamine), TCEP (tris(2-carboxyethyl)phosphine) and biotin azide were add...
