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ProteomicsLactylation of Hdac1 regulated by Ldh prevents the pluripotent-to-2C state conversion
Embryonic stem cells (ESCs) were labeled with heavy isotopes, 13C615N4-arginine (Arg10) and 13C614N2-lysine (Lys6), or light isotopes, 12C614N4-arginine (Arg0) and 12C614N2-lysine (Lys0), respectively, for a total of 7 passages to ensure efficient labeling (>97%). The ESCs labeled with heavy isotope...
ORGANISM(S): Mus Musculus 
PXD050051 |
ProteomicsSelective enrichment of N-terminal proline peptides via hydrazide chemistry for proteomics analysis
A challenge for shotgun proteomics is the identification of low abundance proteins, which is always hampered owing to the extreme complexity of protein digests and highly dynamic concentration range of proteins. To reduce the complexity of the peptide mixture, we developed a novel method to selectiv...
ORGANISM(S): Homo Sapiens 
PXD029178 |
ProteomicsN-glycopeptide Signatures in Liver Diseases
(1) A N-glycopeptide method based on 18O/16O C-terminal labelling to obtain comparisons of serum from patients with HBV-related hepatocellular carcinoma (HCC-O18 labelling) and HBV-related liver cirrhosis (LC-O16 labelling) (2) MRM verification of N-glycopeptide alterations
ORGANISM(S): Homo Sapiens 
2019-09-10 | PXD013586 |
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