A comprehensive analysis of the metabolome, proteome, transcriptome, DNA methylome, and small RNAome profiles of pear fruit flesh at 11 developing stages, spanning from fruitlet to ripening.
Biotinylated proteins were enriched with streptavidin beads followed by on-bead trypsin digestion. Then the LC-MS/MS analysis was performed to identify the biotinylated proteins.
First, Flag-FOXQ1 was overexpressed in HCT-116 cells. Subsequently, the Flag-FOXQ1 complex was enriched via immunoprecipitation (IP) technology. The complex was then washed four times with phosphate-buffered saline (PBS), denatured in loading buffer by boiling, and separated via gel electrophoresis ...
Coronavirus disease 2019 (COVID-19) induces diverse clinical manifestations accompanied by multi-organ dysfunction. Of these, heart complications are the most life threatening. Direct myocardial and vascular injuries due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) drive systemic ...
Through integrative analyses of GWAS summary statistics, population genetic data and expression quantitative trait loci (eQTL) annotations, we found that elevated NEK4 expression in hippocampus was causatively linked to the risk of BD. To examine the biological impact of this gene, we generated NEK4...
