The Staphylococcus aureus strain was cultured in LB medium at 150 rpm and 37 ℃ to a final optical density (OD) of 1.0 at 600 n m. Subsequently, 1 m L of the culture broth was collected and centrifuged at 8000 rpm, 4 ℃ for 2 min. The cell pellet was re-suspended in 2 m L of ice-cold 0.1% (w/v) bacter...
