3289 phosphopeptides were identified and mapped to 1472 proteins that are comparable by phosphoproteomics analysis and annotated in COG/KOG, Domain, KEGG and GO database, respectively
To capture the unknown receptors, we purified recombinant His-tagged FN1859 proteins from Escherichia coli and performed His-tag pull-down assays with WT, KRAS p.G13D and KRAS p.G12D cell lysates. Then the bands submitted for mass spectrometry (MS) protein identification.
