The total proteins from transgenic plants overexpressing Exo70A1 coding sequence in a translational fusion of a GFP-tag were extracted from root stele tissues using a lysis buffer composed of 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5 mM EDTA pH 8.0, 0.5% NP-40, and freshly added 1 mM PMSF, 1 mM DTT, 1...
ORGANISM(S): Zea Mays
