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TranscriptomicssiRIP140 and gene expression effects in E2-treated MCF7 cells
This experiment is designed to assess the role of RIP140 in estrogen receptor-dependent gene expression in MCF7 luminal breast cancer cells. MCF7 cells were transfected with an siRNA to target RIP140 or siControl, and subsequently depleted from hormones for three days. Thereafter, cells were treated...
ORGANISM(S): Homo sapiens 
2015-03-17 | E-MTAB-2577 | biostudies-arrayexpress
GenomicsChIP-Seq for RIP140, using the human breast cancer cell line MCF-7 in the absence of hormone or after treatment with Estradiol
This experiment is designed to assess the role of RIP140 in estrogen receptor-dependent gene expression in MCF7 luminal breast cancer cells. Hormone deprived MCF7 cells were treated for 3 hours with E2 or DMSO control, after which samples were processed for ChIp-seq for RIp140.
ORGANISM(S): Homo sapiens 
2015-03-16 | E-MTAB-2576 | biostudies-arrayexpress
GenomicsGlobal mapping of estrogen receptor and histone modifications in breast tumor samples uncovers distinct patterns for aromatase inhibitor resistance
ChIP-seq on primary breast cancer tumor samples for Era, H3K4me3, H3K27me3. Patients had a poor or good outcome after aromatase inhibitor treatment. Differential binding patterns between good and poor outcome patients were identified for each marker, predicting response to treatment comparing ER, H3...
ORGANISM(S): Homo sapiens 
2013-12-05 | E-GEOD-40867 | biostudies-arrayexpress
GenomicsEpigenetic changes due to histone eviction induced by anthracycline doxorubicin and aclarubicin
One major class of anti-cancer drugs targets topoisomerase II to induce DNA double-strand breaks and cell death of fast growing cells. Here, we compare three members of this class - the antracyclines doxorubicin and aclarubicin, and a chemically unrelated compound, etoposide. Aclarubicin does not i...
ORGANISM(S): Homo sapiens 
2013-06-04 | E-GEOD-33633 | biostudies-arrayexpress
GenomicsAPOBEC3B facilitates a functional and necessary link between estrogen receptor mediated transcription and DNA repair
Oestrogen receptor-α (ER) is the principal transcription factor in the majority of breast cancers, driving expression of genes that control cell growth and endocrine response. Understanding the mechanisms of ER action is crucial for improving response to endocrine treatments. Recent studies show tha...
ORGANISM(S): Homo sapiens 
2015-09-07 | E-GEOD-57426 | biostudies-arrayexpress
GenomicsEstrogen Receptor alpha ChIP-Seq in mouse mammary gland
Estrogen Receptor is a key transcriptional regulator in mammary gland development and breast cancer. In this study, we have mapped the Estrogen Receptor chromatin binding patterns in healthy mouse mammary gland A minimum of 6 pairs of mouse mammary gland pads from mice at 5-6 weeks of age were excis...
ORGANISM(S): Mus musculus 
2013-01-11 | E-GEOD-43415 | biostudies-arrayexpress
GenomicsCo-regulated gene expression by estrogen receptor-a and liver receptor homolog-1 is a feature of the estrogen response in breast cancer cells
Our findings establish a key role for LRH-1 in the regulation of ERa target genes in breast cancer cells and identify a mechanism in which co-operative binding of LRH-1 and ERa at estrogen response elements controls the expression of estrogen responsive g Examination of ERα, with or without LRH-1 kn...
ORGANISM(S): Homo sapiens 
2013-08-01 | E-GEOD-49390 | biostudies-arrayexpress
TranscriptomicsRNA-seq in the U2OS-AR cell line to identify genes regulated by the androgen receptor
To identify the genes regulated by the androgen receptor (AR), we performed RNA-seq in the U2OS-AR cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for human AR), upon androgen (R1881) or vehicle (DMSO) treatment for 24 hours.
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9622 | biostudies-arrayexpress
TranscriptomicsATAC-seq in the U2OS-AR cell line comparing cells treated for 4 hours with 5 nM R1881 to vehicle (DMSO) control-treated cells
We performed ATAC-seq in the U2OS-AR cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for human AR), upon androgen (R1881) or vehicle (DMSO) treatment for 4 hours.
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9606 | biostudies-arrayexpress
TranscriptomicsGenome-wide profiling of H3K27ac, H3K4me1, H3K27me3 and H3K9me3 (ChIP-seq) in U2OS-GR and U2OS-AR cells
We performed ChIP-seq targeting the H3K27ac, H3K4me1, H3K27me3 and H3K9me3 in the U2OS-GR and U2OS-AR cell lines. The cell lines are derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for either rat GR or human AR, respectively. The U2OS-GR cells were treated with dexa...
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9617 | biostudies-arrayexpress
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