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ProteomicsLinking the Vegetative Cell and Spore Proteomes of Peptoclostridium difficile
Peptoclostridium difficile, an anaerobic pathogen, known as causative agent of pseudomembranous colitis and nosocomial diarrhoea. It can also form highly resistant endospores which lay the basic foundations of infection prior germination in the human guts. P. difficile exploits toxins TcdA and TcdB ...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2020-05-26 | PXD012030 | Pride
Proteomics Peptoclostridium difficile Spores One-pot sample processing 
ProteomicsThe influence of sporulation medium on the spore coat protein composition of Bacillus subtilis spores.
Spores are of high interest to the food and health sectors because of their extreme resistance to harsh conditions especially against heat. Earlier research has shown that spores prepared on solid agar plates have a higher heat resistance than those prepared in a liquid medium. It has also been show...
ORGANISM(S): Bacillus subtilis subsp. subtilis 
2017-09-20 | PXD004473 | Pride
Proteomics Sporulation medium Bacillus Quantitative proteomics Spores 
ProteomicsIn culture cross-linking of bacterial cells reveals large scale dynamic protein-protein interactions at the peptide level.
Identification of dynamic protein-protein interactions at the peptide level on a proteomic scale is a challenging approach that is still in its infancy. We have developed a system to cross-link cells directly in culture with the special lysine cross-linker bis(succinimidyl)-3-azidomethyl-glutarate (...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2017-05-23 | PXD006287 | Pride
Escherichia coli Bacillus subtilis Rna polymerase Membrane permeability Transcription. Bamg (bis(succinimidyl)-3-azidomethyl-glutarate) In vivo cross-linking Translation 
Proteomics‘One-pot’ sample processing method for proteome-wide analysis of microbial cells and spores
Bacterial endospores, the transmissible forms of pathogenic bacilli and clostridia, are heterogeneous multilayered structures composed of proteins. These proteins protect the spores against variety of stresses, thus helping spore survival, and assist in germination, by interacting with the environme...
ORGANISM(S): Bacillus cereus (strain ATCC 14579 / DSM 31) Bacillus subtilis PY79 Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2018-07-05 | PXD008242 | Pride
Spores One-pot method Zic-hilic Proteomics Mass spectrometry 
OtherCarbon source-induced reprogramming of the cell wall proteome and secretome modulates the adherence and drug resistance of the fungal pathogen Candida albicans
PXD ID: PXD000008. Original data file: CW1113_q1 GluLac walls rep 2.mgf. Data published as part of Proteomics 2012 12:3164-79 . From Proteomexchange: {{i}}Cell wall and secretome preparation. C. albicans RM1000 cells grown to mid-exponential phase were harvested by centrifugation. The resulting sup...
ORGANISM(S): Candida_albicans 
GPM11210010990 | GPMDB
Resource Reanalysis 
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