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A time-course experiment was performed: HUVECs were exposed for 2, 4, 8 and 24 hours to (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (DETA-NONOate) 250 uM.
ORGANISM(S): Homo sapiens 
HUVEC were exposed to 1 mM sodium nitroprusside (SNP) for 2, 4, 8 and 24 hours and compared to untreated control cells.
ORGANISM(S): Homo sapiens 
HUVEC were exposed to 0.1, 1 and 10 mM sodium nitroprusside (SNP) for 4 hours and compared to untreated control cells.
ORGANISM(S): Homo sapiens 
HUVECs were exposed to 250 uM DETA-NONOate for 4 hours in the presence and absence of 10 uM of the guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo-[4,3,2]quinoxalin-1-one (ODQ). Control cells were exposed to vehicle (methanol 0.1%).
ORGANISM(S): Homo sapiens 
Control samples of HUVEC were obtained parallel with a 4 hr experiment and compared to control.
ORGANISM(S): Homo sapiens 
HUVEC were exposed to 150 minutes of high shear stress using a Cellmax flow cardtridge either in the presence or absence of 100 uM L-NAME or 10 uM L-ODQ; luminal flow was 25 ml/min; resulting a shear stress of 25 dyn/cm2. A simultaneously run cartridge not subjected to luminal, but to pericapillary ...
ORGANISM(S): Homo sapiens 
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