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A common bacterial strategy for monitoring environmental challenges is to use two-component systems, which consist of a sensor histidine kinase (HK) and a response regulator (RR). In the food-borne pathogen Bacillus cereus, the alternative sigma factor σB is activated by the RR RsbY. Here we presen...
ORGANISM(S): Bacillus cereus ATCC 14579 
B. anthracis 7702 and the codY mutant 7CodYS were grown in R-medium (Ristroph, J.D. & Ivins, B.E. (1983). Elaboration of Bacillus anthracis antigens in a new, defined culture medium. Infect. Immun. 39:483-486) with 0.6% sodium bicarbonate until A600 = 0.2 or 1.0. The transcriptome of both strains wa...
ORGANISM(S): Bacillus anthracis 
Comparative phenotype and transcriptome analyses were performed with Bacillus cereus ATCC 14579 exposed to acid down-shock to pH 5.5 set with different acidulants. When acidified with hydrochloric acid (HCl), growth was diminished, whereas 2 mM undissociated lactic acid (HL) or acetic acid (HAc) sto...
ORGANISM(S): Bacillus cereus 
The alternative sigma factor sigmaB has an important role in the acquisition of stress-resistance in many gram-positive bacteria. In the foodborne pathogen Bacillus cereus sigmaB is activated strongly upon a heat shock and other stress conditions. Here we describe the identification of the set of si...
ORGANISM(S): Bacillus cereus ATCC 14579 
In Bacillus cereus the catabolite control protein CcpA was shown to be involved in optimizing the efficiency of glucose catabolism by activating genes encoding glycolytic enzymes including a non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase that mediates conversion of D-glyceraldehyde 3-p...
ORGANISM(S): Bacillus cereus ATCC 14579 
Microarray-based Transposon Mapping (M-TraM) of E. faecium E1162 to identify conditionally essential genes, required for growth in the presence of chlorhexidine (1.2 ug/ml)
ORGANISM(S): Enterococcus faecium 
Two independent aliquots containing approximately 10^7 cells from the transposon mutant of E. faecium E1162 were grown at 37 C for 20 hours in 20 ml of BHI broth. Genomic DNA was isolated from the two replicate cultures and used for the generation of cDNA. The cDNA samples were labeled with Cy3 and ...
ORGANISM(S): Enterococcus faecium 
Mapping of transposon mutant library during growth in Brain Heart Infusion (BHI) broth and BHI broth with 20 ug/ml ampicillin
ORGANISM(S): Enterococcus faecium 
Mapping of transposon mutant library in Enterococcus faecium during growth in Brain Heart Infusion (BHI) broth and in a semi-static biofilm model. The goal of this study was to identify factors that play a role in E. faecium biofilm formation by selection of transposon insertion mutants that lost th...
ORGANISM(S): Enterococcus faecium 
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