Transcriptomic and physiological responses of Bacillus cereus to organic and inorganic acid down-shocks

ABSTRACT: Comparative phenotype and transcriptome analyses were performed with Bacillus cereus ATCC 14579 exposed to acid down-shock to pH 5.5 set with different acidulants. When acidified with hydrochloric acid (HCl), growth was diminished, whereas 2 mM undissociated lactic acid (HL) or acetic acid (HAc) stopped growth without inactivation (bacteriostatic condition), and 15 mM undissociated HAc caused growth arrest and, finally, cell death, as reflected by a 3 to 4 log inactivation (bactericidal condition). Within the first 60 min after pH down-shock, the intracellular ATP levels of cultures shocked with HCl were increased. The bacteriostatic pH shocks did not result in increased nor decreased intracellular ATP levels, indicating that the high energy status within the stressed aerobically grown B.

ORGANISM(S): Bacillus cereus

SUBMITTER: Marcel Tempelaars 

PROVIDER: E-GEOD-15140 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress