Dataset Information

Transcription profiling by array of Arabidopsis wild type and MYB knock out plants

ABSTRACT: Comparing the gene expression patterns between wild type plant (Col-0) and MYB knock-out plants.

PROVIDER: E-ATMX-6 | ExpressionAtlas | 2014-07-03

REPOSITORIES: ExpressionAtlas

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Publications

Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis.

Hirai Masami Yokota MY Sugiyama Kenjiro K Sawada Yuji Y Tohge Takayuki T Obayashi Takeshi T Suzuki Akane A Araki Ryoichi R Sakurai Nozomu N Suzuki Hideyuki H Aoki Koh K Goda Hideki H Nishizawa Osamu Ishizaki OI Shibata Daisuke D Saito Kazuki K

Proceedings of the National Academy of Sciences of the United States of America 20070409 15

Understanding plant metabolism as an integrated system is essential for metabolic engineering aimed at the effective production of compounds useful to human life and the global environment. The "omics" approach integrates transcriptome and metabolome data into a single data set and can lead to the identification of unknown genes and their regulatory networks involved in metabolic pathways of interest. One of the intriguing, although poorly described metabolic pathways in plants is the biosynthes ...[more]

PMID: 17420480

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Project description:Title : Characterization of genes differentially expressed in roots of transgenic arabidopsis lines expressing the p25 protein of beet necrotic yellow vein virus. Biological question : Rhizomania ("crazy root") is a severe disease of sugar beet caused by beet necrotic yellow vein virus (BNYVV), which is transmitted by the soil-inhabiting fungus Polymyxa betae. Symptoms of virus infection are characterized by a constricted tap root and a massive proliferation of fine rootlets that often undergo necrosis. BNYVV RNA-3 encodes a 25 kDa (p25) which is an important determinant of leaf symptom phenotype. It also governs BNYVV invasion of the plant root system and induction of rootlet proliferation in sugar beet. In order to obtain a better understanding of molecular aspects of disease development in roots and to characterize specific host genes involved in response to viral infection, transgenic Arabidopsis overexpressors of p25 viral protein was obtained and better characterized. It was shown that transgenic plants that efficiently expressed p25 protein produced more lateral roots. Comparative analysis (microarray) was performed between wild type Arabidopsis roots and transgenic Arabidopsis roots expressing p25 protein, in order to identify Arabidopsis genes differentially expressed in response to p25 viral protein. Experiment description: Seeds were surface sterilized, chilled at 4C for 4 days, and then germinated and grown on square Petri plates containing sterilized Murashige and Skoog (MS) medium with 1% sucrose. Such stock plates were arranged vertically in plastic racks and placed into growth chamber. After 5 days, plants were transferred carefully onto fresh MS medium big round plates. On each plate, 60 Wild Type (WT) plantlets were transferred on the half right of the plate, and 60 transgenic plantlets (B, E or T lines) were transferred on the half left of the plate. Plates were arranged horizontally and placed into growth chamber. Experiment 1 : 5 plates containing WT0A control plants and B0A transgenic plants. Experiment 2 : 5 plates containing WT1 control plants and B transgenic plants. 5 plates containing WT2 control plants and E transgenic plants. 5 plates containing WT3 control plants and T transgenic plants. Plants were harvested after 7 days (experiment 1) or 12 days (experiment 2), and WT roots or transgenic roots were pooled and conserved at -80C.

Dataset Information

Transcription profiling by array of Arabidopsis wild type and MYB knock out plants

Publications

Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis.

Similar Datasets

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