Proceedings of the National Academy of Sciences of the United States of America 20070409 15
Understanding plant metabolism as an integrated system is essential for metabolic engineering aimed at the effective production of compounds useful to human life and the global environment. The "omics" approach integrates transcriptome and metabolome data into a single data set and can lead to the identification of unknown genes and their regulatory networks involved in metabolic pathways of interest. One of the intriguing, although poorly described metabolic pathways in plants is the biosynthes ...[more]
Project description:Homeostasis of histone acetylation and the control of transcription. Involvement of histone acetyl transferase HAG4 in the root development.<br> hag4 mutant (with a insertion in HAG4 gene encoding a Histone Acetyl Transferase) and wild-type ecotype (Ws) were grown during 15 days, in vitro. RNA were extrated from roots of seedlings. Each sample (ws or hag4) corresponds to a pool of 3 independant cultures and harvesting. <br>
Project description:Arabidopsis plants are grown for 5 days on MS medium and then we transfer them to a medium supplemented with ACC (5uM) or without ACC. After 3 h of growth, we isolate the RNA of the roots.
Project description:Identification of new genes regulated by RDR6 and SGS3 (two genes involved in PTGS) by analysis of the transcriptome of rdr6-1 and sgs3-1 mutants compared to wild-type plants in different tissues (flower and leaves). The comparison between transcriptome of rdr6-1 and sgs3-1 mutant alleles impaired in PTGS and development (juvenile-to-adult transition) and transcriptome of rdr6-5 and sgs3-3 alleles impaired only in PTGS would allowed identification of genes involved in the developmental default (zip phenotype) of the null alleles (rdr6-1 and sgs3-1 mutants).