Dataset Information


Gene expression profiles of mouse embryonic stem cell derived macrophages infected with Salmonella typhimurium

ABSTRACT: This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see established protocols to generate monocytes and macrophages from murine embryonic stem cell (129 background) in vitro. Briefly, murine embryonic stem cells were grown in bacteriological dishes in the presence of differentiation medium supplement ed with 15% L929 and 1ng/ml IL-3 for 8 days to generate embryoid bodies (EBs). Subsequently, EBs were transferred to gelatinized tissue culture dish. Supernatants containing macrophage progenitors were collected, spun down and plated onto bacteriological dishes to differentiate into macrophages. Four biological replicates of murine embryonic stem cells, macrophage progenitors and macrophages were collected and their RNA were isolated via the Qiagen RNA Isolation Kit. The RNA were treated with RNAse free DNase and eluted in RNase-free water. THe RNA concentration was assessed using a NanoDrop spectrophotometer, while RNA integrity (RIN >8) were obtained for all samples by Agilent 2100 Bioanalyzer. We aim to sequence 5GBp per replicate and 4 replicates per lane on an Illumina platform.


ORGANISM(S): Mus musculus  

PROVIDER: E-ERAD-132 | ExpressionAtlas | 2016-06-07

REPOSITORIES: ExpressionAtlas

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Conditional-ready mouse embryonic stem cell derived macrophages enable the study of essential genes in macrophage function.

Yeung A T Y AT   Hale C C   Xia J J   Tate P H PH   Goulding D D   Keane J A JA   Mukhopadhyay S S   Forrester L L   Billker O O   Skarnes W C WC   Hancock R E W RE   Dougan G G  

Scientific reports 20150310

The ability to differentiate genetically modified mouse embryonic stem (ES) cells into functional macrophages provides a potentially attractive resource to study host-pathogen interactions without the need for animal experimentation. This is particularly useful in instances where the gene of interest is essential and a knockout mouse is not available. Here we differentiated mouse ES cells into macrophages in vitro and showed, through a combination of flow cytometry, microscopic imaging, and RNA-  ...[more]

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