Project description:Primary human hepatocytes were treated with compounds modulating steatosis: palmitic acid, compound C and metformin qPCR miRNA expression profiling. Hepatocytes were treated as indicated in the summary. Equal amount total RNA was pooled prior to miRNA expression analysis

2020-02-11 | GSE145039 | GEO

Homo sapiens

Project description:Primary hepatocytes with glucagon treatment expression profiling

| PRJNA282088 | ENA

Protein data in PHH after VPA-treatment

Project description:LC-MS/MS protein data of Primary Human Hepatocytes (PHH) exposed to Valproic Acid (VPA) for 3 days daily and 3 days daily exposure followed by 3 days washout.

2018-02-27 | PXD007538 | Pride

Induction of changes in primary human hepatocytes by valproic acid

Project description:Induction of changes in primary human hepatocytes by valproic acid

| PRJNA328467 | ENA

Genome wide binding and transcriptome analysis of human FXR in primary human hepatocytes [HTS]

Project description:We report the genome-wide profiling of FXR binding by ChIP-seq from GW4064 or DMSO treated primary human hepatocytes. We reported altered RNA expression profiles in primary human hepatocypes upon GW4064 treatment compared to DMSO control by RNA-seq. We also reported the altered RNA expression profiles in livers from WT C57BL/6J mice upon GW4064 treatment compared to vehicle control. Primary human hepatocytes were treated with 5uM GW4064 or DMSO control, 1 hour later, cells were fixed and collected for chromatin isolation. 24 hours post treatment, cells were isolated for RNA isolation. This submission represents HTS component of study.

2014-07-24 | E-GEOD-57227 | biostudies-arrayexpress

Comparative gene expression profiles induced by PPARM-NM-3 and PPARM-NM-1/M-NM-3 agonists in human hepatocytes

Project description:Transcriptomic profiling of primary human hepatocytes after treatment with 2 glitazones (TRO and ROSI) and 2 glitazars (MURA and TESA) Singe-Channel Samples: We have analysed changes in gene expression profiles in primary human hepatocytes after a 24 h treatment with various concentrations of glitazones and glitazars Dual-Channel Samples: We have analysed changes in gene expression profiles in primary human hepatocytes and differentiated human hepatoma HepaRG cells after a 24 h treatment with various concentrations of glitazones and glitazars Singe-Channel Samples: 5 condition experiments, control, TRO (3 concentrations) , ROSI (3 concentrations) , MURA (3 concentrations) and TESA (1 concentration) , biological replicates:2 to 4 Dual-Channel Samples: 5 condition experiments, control, TRO (3 concentrations) , ROSI (4 concentrations) , MURA (4 concentrations) and TESA (1 concentration) , biological replicates: 3

2014-02-09 | E-GEOD-27183 | biostudies-arrayexpress

Single-cell transcriptomics reveals heterogeneous metabolic profiles in primary human hepatocytes

Project description:Through scRNA-sequencing of primary human hepatocytes (PHHs), we have identified four subgroups of hepatocytes. A phenotyping 5-probe cocktail (Sanofi-Aventis) has been used to assess their metabolic capacity. Upon cocktail treatment, the characterized four hepatocyte subgroups displayed differential gene expression profiles and exhibited xenobiotic metabolism-related specialization. Intracellular lipid accumulation achieved through loading the cells with free fatty acids (FFA, 2:1 oleic:palmitic acid), differently affected the four subgroups. Moreover, we have shown that intracellular fat accumulation diminishes the drug-related metabolic capacity of hepatocytes.

2023-09-19 | E-MTAB-11530 | biostudies-arrayexpress

Homo sapiens

Project description:Aberrantly microRNA profiling in cancerous hepatocytes versus normal primary hepatocytes

| PRJNA124197 | ENA

Genome wide binding and transcriptome analysis of human FXR in primary human hepatocytes [HTS]

2014-07-24 | GSE57227 | GEO