ABSTRACT: Transcription profiling by array of human umbilical vein endothelial cells (HUVEC) stimulated with TNF vs. controls reveals TNF induces distinct expression programs
Project description:We have identified that VCAM1 is heterogeneously expressed in human umbilical vein endothelial cells (HUVEC). In this experiment, we aimed to identify genes which are regulated by TNF and genes which are differentially regulated in the highest and lowest 5% VCAM1 expressing cells.
Project description:Endothelial cell secretomes were analyzed using culture medium derived from control young and premature senescent human umbilical vein endothelial cell (HUVEC).
Project description:In the hematopoietic microenvironment, endothelial cells (ECs) play an important role in the regulation of hematopoietic cell proliferation and trafficking. We previously demonstrated that EC stimulated with tumor necrosis factor alpha (TNF-α) induce the generation of dendritic cells from CD34(+) stem cells, whereas in contrast, interleukins were capable of inducing the proliferation of hematopoietic and myeloid progenitors. In order to identify potentially new soluble factors which greatly impact the self-renewal, proliferation and differentiation of CD34+ hematopoietic stem cells (HSC), we examined the expression profiles of IL-1ß, IL-3 and IL-6 stimulated human umbilical vein endothelial cells (HUVEC).
Project description:In the hematopoietic microenvironment, endothelial cells (ECs) play an important role in the regulation of hematopoietic cell proliferation and trafficking. We previously demonstrated that EC stimulated with tumor necrosis factor alpha (TNF-α) induce the generation of dendritic cells from CD34(+) stem cells, whereas in contrast, interleukins were capable of inducing the proliferation of hematopoietic and myeloid progenitors. In order to identify potentially new soluble factors which greatly impact the self-renewal, proliferation and differentiation of CD34+ hematopoietic stem cells (HSC), we examined the expression profiles of IL-1ß, IL-3 and IL-6 stimulated human umbilical vein endothelial cells (HUVEC). we processed seven different umbilical cords and isolated 129 samples for total RNA, and pooled them into 18 groups corresponding to each stimulant, control and time point.
Project description:Human umbilical vein endothelial cells (HUVECs) expressing vPK (HUVEC-vPK) have a survival advantage over control HUVEC under conditions of extrinsic- and intrinsic-mediated apoptosis.
Project description:We have identified a set of endothelial-enriched long noncoding RNAs (lncRNAs) in another experiment. In this experiment, we aim to identify genes that are regulated by the GATA2-AS1 lncRNA. We have depleted GATA2-AS1 using two distinct siRNA in human umbilical vein endothelial cells (HUVEC)
Project description:We describe the transcriptional response to infection of human umbilical vein endothelial cells (Huvec) with different rubella virus strains
Project description:Transcriptional profiling of Human Umbilical Vein Endothelial Cells (HUVEC) comparing untreated control cells with IL-1-treated cells with or without pre-treatment with DHA. Three condition experiment: DHA treated HUVEC cells (25 µmol/L DHA for 48 hours) vs control; IL-1 treated HUVEC cells (5ng/ml for 3 hours) vs control; HUVEC treated with 25 µmol/L DHA for 48 hours and then stimulated with 5 ng/mL IL-1? for 3 hours. For each condition, 3 replicates
Project description:To investigate sex differences in endothelial cell response to AngII, male and female human umbilical vein endothelial cells (HUVEC) were treated with AngII for 24 hours. RNA seq was run, and demonstrated that female HUVEC had elevated genes associated with oxidative stress and inflammatory responses compared to male HUVEC following AngII treatment.