Project description:Purpose: The goal of this study is to compare NGS-derived Arabidopsis thaliana transcriptome profiling (RNA-Seq) obtained from tissues infected with a variety of Pseudomonas syryingae effector mutants to better understand their impact on their host's transcriptional program. We report the application of RNA-sequencing technology (Illumina Hi-Seq) for high-throughput profiling Arabidopsis thaliana transcriptomes upon infection with the model pathogen Pseudomonas syringae (wild-type and mutants).
Project description:Here we use bisulfite conversion of rRNA depleted RNA combined with high-throughput Illumina sequencing (RBS-seq) to identify single-nucleotide resolution of m5C sites transcriptome-wide in Arabidopsis thaliana seedlings. m5C sites were also analyzed in Arabidopsis trm4b-1 and trdmt1 T-DNA KO mutants for the RNA methyltransferases TRM4B and TRDMT1.
Project description:In order to dissect the roles of sequence-specific transcription factors WRKY25, WRKY33 and WRKY75 in apoplastic ROS signaling, a transcriptome profiling of ozone response was done using two arabidopsis mutants: a double mutant wrky25 wrky33 and a single mutant wrky75. The single mutant lines used for transcriptomic analyses were obtained from NASC: wrky25 (SAIL_529_B11) and wrky33 (SALK_006603) were crossed to generate double mutant wrky25 wrky33. T-DNA insertion mutant wrky75-25 (N121525) was obtained from NASC.
2016-04-03 | GSE79855 | GEO
Project description:Arabidopsis stn7 and stn8 single and double mutants vs WT
Project description:Comparison of the endogenous small RNA content of Arabidopsis flower bud tissue: wild type vs. mutants in polIV pathways Keywords: High throughput 454 small RNA sequencing.
Project description:Part of a set of highly integrated epigenome maps for Arabidopsis thaliana. Keywords: Illumina high-throughput transcriptome sequencing Transcriptome sequencing of wildtype Arabidopsis plants (Columbia-0), and met1, drm1 drm2 cmt3, and ros1 dml2 dml3 null mutants using the Illumina Genetic Analyzer.
Project description:Part of a set of highly integrated epigenome maps for Arabidopsis thaliana. Keywords: Illumina high-throughput bisulfite sequencing Whole genome shotgun bisulfite sequencing of wildtype Arabidopsis plants (Columbia-0), and met1, drm1 drm2 cmt3, and ros1 dml2 dml3 null mutants using the Illumina Genetic Analyzer.
Project description:Part of a set of highly integrated epigenome maps for Arabidopsis thaliana. Keywords: Illumina high-throughput small RNA sequencing Small RNA sequencing of wildtype Arabidopsis plants (Columbia-0), and met1, drm1 drm2 cmt3, and ros1 dml2 dml3 null mutants using the Illumina Genetic Analyzer.
Project description:Transcriptional profiling of WT, cytosolic ascorbate peroxidase 1 (Apx1) and peroxisomal catalase 2 (Cat2) single mutants and apx1/cat2 double mutant plants, subjected to 0h and 1h of high light irradiation in order to identify molecular mechanisms underlying the observed growth retardation and stress resistance of apx1/cat2 double mutant plants. 4 genotypes x 2 conditions experiment including WT, apx1, cat2, and apx1/cat2 plants treated with 0h and 1h of high light irradiation. Two biological replicates. In total, 16 samples hybridized on 8 Arabidopsis Gene Expression microarrays V3 (Agilent, two-color array) including color swap of replicate samples.
Project description:BRD1, BRD2, and BRD13 are likely subunits of Arabidopsis SWI/SNF chromatin remodeling complexes. To investigate the effect of disruption of BRD genes on global gene expression in Arabidopsis, we performed transcriptome profiling of 18-day-old single brd2, double brd1 brd2 and triple brdx3 mutants.