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E-GEOD-39194

ABSTRACT: Effect of ATF4 overexpression on mRNA levels in differentiated C2C12 myotubes.

PROVIDER: E-GEOD-39194 | ExpressionAtlas | 2015-09-29

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-39194-analysis-methods.tsv	Tabular
	E-GEOD-39194-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-39194-configuration.xml	Xml
	E-GEOD-39194-percentile-ranks.tsv	Tabular
	E-GEOD-39194.condensed-sdrf.tsv	Tabular

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Project description:ATF4 is a bZIP transcription factor that that promotes skeletal muscle atrophy. The goal of these studies was to determine the effects of ATF4 overexpression on mRNA levels in differentiated C2C12 myotubes. For additional details see Ebert et al, Stress-Induced Skeletal Muscle Gadd45a Expression Reprograms Myonuclei and Causes Muscle Atrophy. JBC epub. June 12,2012 C2C12 myotubes were infected with adenovirus co-expressing eGFP and ATF4-FLAG. Control myotubes were infected with adenovirus co-expressing eGFP and a transcriptionally inactive ATF4 construct (ATF4∆bZIP).

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Effect of ATF4 overexpression on mRNA levels in differentiated C2C12 myotubes.

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Project description:Gadd45a is a stress-induced protein that causes skeletal muscle atrophy. The goal of these studies was to determine the effects of Gadd45a overexpression on mRNA levels in mouse skeletal muscle. For additional details see Ebert et al, Stress-Induced Skeletal Muscle Gadd45a Expression Reprograms Myonuclei and Causes Muscle Atrophy. JBC epub. June 12, 2012. Tibialis anterior (TA) muscles from muscle-specfic ATF4 knockout mice (ATF4 mKO) were transfected with either 20 mg empty plasmid (pcDNA3) (left TA) or 20 mg pCMV-FLAG-Gadd45a (right TA) and harvested 7 days later. mRNA levels in Gadd45a-transfected muscles were normalized to levels in control transfected muscles.

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