ABSTRACT: RNA-seq of coding RNA from 9-day old Arabidopsis seedlings of link1link2 double mutants against wild-type controls grown under continuuous light or long day conditions and sampled across a 24-hour time-course
Project description:au10-03_hub1 - kinetics of gene expression after light exposure in hub1-3 mutant compared to wild-type - Role of histone UbH2B in the regulation of gene expression in response to light - RNA from 5-day-old wild-type and hub1-3plants in 3 conditions were used for hybridization: 5 days of darkness, 5 days of darkness + 1 hour of light and 5 days of darkness + 6 hours of light. Keywords: genotype comparaison,time course
Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of wild type plants with that of link1link2 double mutants grown under continous light or long day (16h light/8 h darkness) conditions. In the first experiment wild-type and link1link2 mutant plants were grown for two weeks under continuous white light conditions at 22 degrees centigrades and the transcriptional profile of these plants was analyzed using RNA-seq. In the second experiment, wild-type and link1link2 mutants were grown under long day conditions (16 h light/8 h darkness), and the transcriptional profile of these plants was analyzed in samples harvested every 4 hours over a 24 hour time-course, starting 2 hours after the onset of the light period.
Project description:This is a 24 hour time course experiment to investigate changes in gene expression in deseeded berries sampled every three hours within a 24 hour period at 2 time points during development i.e. T4 (preveraison) & T15 (postveraison). Changes in gene expression within the 24 hour period were analyzed to identify expression profiles that coincided with diurnal changes. The gene expression profiles were then to be compared between the two developmental time points T4 v T15). Keywords: Diurnal time course
Project description:This is a 24 hour time course experiment to investigate changes in gene expression in deseeded berries sampled every three hours within a 24 hour period at 2 time points during development i.e. T5 (preveraison) & T17 (postveraison). Changes in gene expression within the 24 hour period were analyzed to identify expression profiles that coincided with diurnal changes. The gene expression profiles were then to be compared between the two developmental time points T5 v T17). Keywords: Diurnal time course
Project description:au10-03_hub1 - kinetics of gene expression after light exposure in hub1-3 mutant compared to wild-type - Role of histone UbH2B in the regulation of gene expression in response to light - RNA from 5-day-old wild-type and hub1-3plants in 3 conditions were used for hybridization: 5 days of darkness, 5 days of darkness + 1 hour of light and 5 days of darkness + 6 hours of light. Keywords: genotype comparaison,time course 10 dye-swap - CATMA arrays
Project description:The 19S proteasome mutant rpn12a-236 exhibits a stay-green phenotype upon individually darkened leaf treatment (IDL). To gain insight into the molecular mechanisms conferring this extended longevity to rpn12a-236 in response to darkness, we performed RNA-seq analysis on WT and mutant leaves sampled after 0 hour (light control), 6 hours, 1 day, 3 days and 6 days of IDL treatment.
Project description:Male C57Bl/6 mice were randomized to undergo 5 days of i) a shiftwork protocol (10-hour light: 10-hour dark cycle) before myocardial infarction (MI) surgery, ii) a normal 12-hour light: 12-hour dark environment before MI surgery, iii) a normal 12-hour light: 12-hour dark environment and used as sham controls, or iv) a shiftwork protocol (10-hour light: 10-hour dark cycle) and used as sham controls. MI surgery was performed on the 5th day, after which all mice were returned to a normal 12-hour light: 12-hour dark cycle. Hearts were collected 24-hours post-MI at ZT06. The microarray approach allows the investigation of transcriptome-wide gene expression changes in hearts from mice on a shiftwork cycle or on a regular light:dark cycle before MI.
Project description:Eleven healthy human subjects were enrolled in a 6-day simulated shift work protocol. Blood samples were collected during the two 24-hour measurement periods. Blood samples were collected every 4 hours during both measurement periods. Subjects entered the lab on Day 1. At the start of Day 2, the first 24-hour measurement period was started. Subjects slept according to their habitual sleep/wake schedule, followed by a 16-hour constant posture procedure. On days 3-6, the sleep period was delayed by 10 hours. Participants in the control group were exposed to dim light throughout the waking periods; participants in the bright group were exposed to bright light (~6,000lux) for 8 hours during the waking period. Following the third night on this schedule, subjects underwent another 24-hour measurement period. During both measurement periods, blood samples were collected and PBMCs were isolated. mRNA was extracted, labelled, and hybridized to microarrays.
Project description:Overall, the study aims at obtaining a comprehensive picture of the African malaria mosquito, Anopheles gambiae, transcriptome using high-coverage RNA-seq of sexed whole-insect samples collected at different developmental time points. This experiment focuses on male and female transcriptomes from 20 hour old embryos, 12 hour old 3rd instar larvae, 24 hour old 4th instar larvae and 10 hour old pupae sampled using a strand-specific RNA-seq approach.