Project description:rs09-07_hb6 - hb6 - The aim is to determine downstream target of HB6 transcription factor involved in plant development - Transcriptome on Arabidopsis HB6 over-expressing plants and BPM Knock down plants during development Keywords: wt vs mutant comparison
Project description:Microarrays were used to detail the global programme of gene expression comparing wild-type and RNAi knock-down plants of SPT4-1 and SPT4-2
Project description:rs04-09_hahb4 - water stress - We want to know which genes are over-expressed or down regulated in Arabidopsis plants transformed with the sunflower hahb-4 homeodomain transcription factor with respect to non transformed ones in control and water stress conditions. This gene, hahb4, confers drought tolerance to transgenic Arabidopsis plants. - Overexpression of the hahb4 gene vs vector transformed control Keywords: gene knock in (transgenic),treated vs untreated comparison
Project description:gen107_ptgs - gene profiling in silencing suppressor plants or in mirna mutants - What are the genes that are differentially regulated in various tissues derived from silencing suppressor plants or miRNA mutants? - HcPro, P15, P19, CHS-RNAi (control), were grown on MS solid medium, and tissues were harvested at different developmental stages. dcl1-9, hen1-1 and La-er (control) were grown on MS solid medium, and tissues harvested at different developmental stages. Keywords: gene knock in (transgenic),gene knock out 40 dye-swap - Chromochip Arabidopsis thaliana 21.7K CHROMO4_1
Project description:rs09-07_hb6 - hb6 - The aim is to determine downstream target of HB6 transcription factor involved in plant development - Transcriptome on Arabidopsis HB6 over-expressing plants and BPM Knock down plants during development Keywords: wt vs mutant comparison 6 dye-swap - CATMA arrays
Project description:We investigated the role of HSFA1a, a master regulator of heat stress response, in this reorganization through promotion of the formation of promoter/enhancer chromatin loops. We performed gene expression profiling analysis using data obtained from RNA-seq of 4 weeks old tomato (Solanum lycopersicum cv. Moneymaker background ) plants (wild type and RNAi knock-down lines) which were treated at 45°C for 0h, 1h and 6h.
Project description:rs04-09_hahb4 - water stress - We want to know which genes are over-expressed or down regulated in Arabidopsis plants transformed with the sunflower hahb-4 homeodomain transcription factor with respect to non transformed ones in control and water stress conditions. This gene, hahb4, confers drought tolerance to transgenic Arabidopsis plants. - Overexpression of the hahb4 gene vs vector transformed control Keywords: gene knock in (transgenic),treated vs untreated comparison 3 dye-swap - CATMA arrays
Project description:The plant vascular system is essential for the enlarged plant stature and successful colonizzation the land by delivering resources throughout the plants and providing mechanical support. Despite several regulators of vascular patterning have been reported, how vascular system mediates stress resistance remain largely unknown. Here we identified a CsIND transcription factor that is specifically expressed in the xylem and phloem tissues in cucumber. Knock down of CsIND by RNAi lead to dwarf plants with enlarged or disorganized vascular systems in all aerial organs. The content of both auxin and jasmonic acid were increased in the CsIND-RNAi lines. Transcriptome profiling by RNA-Seq hints CsIND-regulated gene networks for defense response and vascular development. Biochemical analyses verified that CsIND directly binds to well-known vascular regulators including CsCCR1, CsMYB116, CsYAB5, CsBP and CsAUX, and physically interacts with dorsiventral patterning genes CsKAN2 and CsYAB5. Further, CsIND-RNAi plants displayed significantly enhanced tolerance to nitrogen dificency and resistance to cucumber downy mildew. Therefore, CsIND regulates vascular formation and resistance to biotic and abiotic stresses in cucumber, through the combinarory interactions with well-known vascular regulaors and hormone metabolism and signaling pathways.