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Expression data from MTF mutant roots

ABSTRACT: Mutation of MTF in Arabidopsis increases Agrobacterium-mediated transformation susceptibility. Being a putative transcription factor, different genes controlling transformation may be regulated by MTF. We used microarrays to detail the global programme of gene expression and to identify genes that play a role in transformation. Root samples from mutants MTF1-1 and MTF1-4, along with the wild-type, Col0, were collected after 12 days of growth in B5 liquid medium. RNA was extracted from three biological replicates, with 20 plants in each replicate, and used for hybridization to Affymetrix ATH1 microarrays.

OTHER RELATED OMICS DATASETS IN: PRJNA221784

PROVIDER: E-GEOD-51236 | ExpressionAtlas | 2015-05-11

REPOSITORIES: ExpressionAtlas

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Cytokinins secreted by Agrobacterium promote transformation by repressing a plant myb transcription factor.

Sardesai Nagesh N Lee Lan-Ying LY Chen Huabang H Yi Hochul H Olbricht Gayla R GR Stirnberg Alexandra A Jeffries Jacob J Xiong Kia K Doerge R W RW Gelvin Stanton B SB

Science signaling 20131119 302

Agrobacterium-mediated transformation is the most widely used technique for generating transgenic plants. However, many crops remain recalcitrant. We found that an Arabidopsis myb family transcription factor (MTF1) inhibited plant transformation susceptibility. Mutating MTF1 increased attachment of several Agrobacterium strains to roots and increased both stable and transient transformation in both susceptible and transformation-resistant Arabidopsis ecotypes. Cytokinins from Agrobacterium tumef ...[more]

PMID: 24255177

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Expression data from MTF mutant roots

Project description:Mutation of MTF in Arabidopsis increases Agrobacterium-mediated transformation susceptibility. Being a putative transcription factor, different genes controlling transformation may be regulated by MTF. We used microarrays to detail the global programme of gene expression and to identify genes that play a role in transformation. Root samples from mutants MTF1-1 and MTF1-4, along with the wild-type, Col0, were collected after 12 days of growth in B5 liquid medium. RNA was extracted from three biological replicates, with 20 plants in each replicate, and used for hybridization to Affymetrix ATH1 microarrays.

2013-11-14 | E-GEOD-51236 | biostudies-arrayexpress

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Expression profiles in two early developmental stages of Sparus aurata

Project description:Two different early developmental stages of gilthead sea bream: i) larvae at 24 hours post-hatching ( Stage 1), and ii) larvae at 96 hours post-hatching (Stage 4), were used for gene expression analysis. For each stage, total RNA was extracted from five (5) independent biological replicates, each consisting of pools of approximately 40-50 larvae. Based on SAM analysis, 1518 genes were differentially expressed between the two stages with a FDR (False Discovery Rate) of 0.0.

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Pof D119 mutant expression analysis

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