Dataset Information


Gene regulation in denervated hairy skin of the adult rat

ABSTRACT: This study aimed to quantify the regulation of transcripts in the hairy skin of the back of adult rats in the condition of loss of sensory and autonomic (sympathetic) innervation (i.e., denervated). Denervated skin has reduced wound healing capacity, reduced proliferation of epidermal progenitor cells, and also expresses factors that regulate ingrowth of sensory and sympathetic axons from neighboring regions of innervated skin. It was expected that this quantification f transcript regulation would offer insight into the general and specific mechanisms that may contribute to these important biological processes. All animals were adult (200-250g) Sprague-Dawley female rats. Three conditions were examined. Groups were naive (n=6), 7-day denervated skin (n=5), and 14-day (n=5) denervated skin. Denervation preparations: Full-thickness incision along long-axis of the body 1cm to right of midline (to avoid injuring skin to be sampled). Incision was centered rostro-caudally on the T13 (thoracic 13) costo-vertebral angle so as to allow access to the T9-L2 (lumbar 2) cutaneous nerves. Skin was reflected to the left and the left T9, T10, L1, and L2 dorsal and lateral cutaneous nerves were isolated, ligated with 7-0 monofilament suture close to their exit from the body wall, and transected. Approximately 5mm of the distal portion of the nerve was resected. The T11 nerves were left unperturbed and were not isolated from the surrounding fascia. This generated two strips of skin that were devoid of sensory and autonomic innervation (those strips served by the T9 and T10 nerves, and by the L1 and L2 nerves). Between these denervated strips of skin was a strip of skin that retained the sensory and autonomic axons supplied by T11 nerves. The denervated zones were identified by mapping the cutaneous trunk muscle (CTM) reflex (see Petruska-JC et al. (2013) Journal of Comparative Neurology; Diamond-J et al., (1992) J Neuroscience), and the border marked with pen and remarked every few days. The samples were taken from the rostral (T9/10) denervated zone. Samples from naive animals (no denervation) were taken from the same region, using the dorsal cutaneous nerves as registration landmarks. Animals displayed no signs of overgrooming of the denervated skin. Because the CTM inserts onto the dermis of this region of skin, samples necessarily include both skin and underlying CTM (which is innervated from another source so was not denervated in the experiment).


ORGANISM(S): Rattus norvegicus  

PROVIDER: E-GEOD-54356 | ExpressionAtlas | 2015-07-31

REPOSITORIES: ExpressionAtlas

altmetric image


categoryCompare, an analytical tool based on feature annotations.

Flight Robert M RM   Harrison Benjamin J BJ   Mohammad Fahim F   Bunge Mary B MB   Moon Lawrence D F LD   Petruska Jeffrey C JC   Rouchka Eric C EC  

Frontiers in genetics 20140429

Assessment of high-throughput-omics data initially focuses on relative or raw levels of a particular feature, such as an expression value for a transcript, protein, or metabolite. At a second level, analyses of annotations including known or predicted functions and associations of each individual feature, attempt to distill biological context. Most currently available comparative- and meta-analyses methods are dependent on the availability of identical features across data sets, and concentrate  ...[more]

Similar Datasets

2014-04-08 | E-GEOD-54356 | ArrayExpress
2018-01-01 | S-EPMC6561483 | BioStudies
1000-01-01 | S-EPMC3101194 | BioStudies
2016-03-01 | E-GEOD-72551 | ArrayExpress
1000-01-01 | S-EPMC3806913 | BioStudies
2013-01-01 | S-EPMC3753026 | BioStudies
1000-01-01 | S-EPMC4843916 | BioStudies
1000-01-01 | S-EPMC5722367 | BioStudies
2018-01-01 | S-EPMC6135988 | BioStudies
2019-01-01 | S-EPMC6511030 | BioStudies