Project description:We generated HiC data from sorted mesodermal and non-mesodermal cells from stage 11-12 Drosophila embryos to explore the distribution of genomic contacts of silencers and non-silencers (identified elsewhere in the manuscript).
Project description:To search for the functional “m6A readers” specifically in the context of Drosophila early embryos, we incubated m6A-modified or unmodified RNA probes with cytosolic lysates from early embryos, and performed immunoprecipitation experiments followed by mass spectrometry analysis. We found that Drosophila FMRP (FMR1) was highly abundant in complexes immuno-precipitated by the m6A-modified probe. We performed data-independent acquisition (DIA) quantitative proteomics on the wild type and fmr1 maternal mutant embryos at multiple developmental stages, including 0-1 h, 2-3 h, and 5-6 h stages. To screen the FMR1-associated partners, we collected the 2–3-hour embryos and performed immunoprecipitation using the anti-Drosophila FMR1 antibody, followed by mass spectrometric analysis.
Project description:In order to identify Ladybird mesodermal and heart target genes, we decided to analyse the transcriptional profiling of embryos with a targeted gain and Loss of function approach. Embryos were collected at stages 11 to 15 of embryogenesis corresponding to the lb expression period. Homozygous ectopically expressing or repressing embryos were collected from a large scale cross of UAS-lbe or UAS-RNAi-lbe flies with 24B-Gal4 or Tin-Gal4 flies, in parallel to stage-matched control. Three independent collections were performed. Total RNA was extracted. Ectopically expression and reference samples were hybridized together on GeneChip Drosophila Genome array2. With this approach, we were able to dicover a repertory of genes involved in different aspects of muscle and heart formation showing a multi-step action of this family of identity genes.
Project description:miRNAs are not well known their expression and function in tooth development. To identify the miRNAs expression during tooth development, tooth germs were dissected from the initiation bud, cap and bell stages. miRNA-chip expression analysis was performed with RNAs of the molar tooth germs from embryos of pregnant mice at emrbryonic day 11, 12, 14, and 16, using Agilent's miRNA microarray.
Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains ChIP-chip data on Agilent 244K dual-color arrays H3K4me1 at 12 different time-points of Drosophila development. Current Dataset: [GSM386042..GSM386050]: ChIP-chip of H3K4Me1 in Drosophila embryos at 4-8 hours of development [GSM386051..GSM386059]: ChIP-chip of H3K4me1 in Drosophila embryos at 12-16 hours of development [GSM386060..GSM386068]: ChIP-chip of H3K4me1 in Drosophila embryos at 16-20 hours of development [GSM386069..GSM386077]: ChIP-chip of H3K4me1 in Drosophila embryos at 20-24 hours of development [GSM418275..GSM418283]: ChIP-chip of H3K4me1 in Drosophila embryos at 8-12 hours of development [GSM442224..GSM442232]: ChIP-chip of H3K4me1 in Drosophila embryos at 0-4 hours of development [GSM442233..GSM442241]: ChIP-chip of H3K4me1 in Drosophila Pupae [GSM442242..GSM442250]: ChIP-chip of H3K4me1 in Drosophila L3 [GSM442251..GSM442259]: ChIP-chip of H3K4me1 in Drosophila L2 [GSM442260..GSM442268]: ChIP-chip of H3K4me1 in Drosophila L1 [GSM442269..GSM442277]: ChIP-chip of H3K4me1 in Drosophila Adult male [GSM442278..GSM442286]: ChIP-chip of H3K4me1 in Drosophila Adulat female For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains ChIP-chip data on Agilent 244K dual-color arrays for antibody: H3K27me3 at 12 different time-points of Drosophila development. Current Dataset: [GSM384769..GSM384777]: ChIP-chip of H3K27me3 in Drosophila embryos at 0-4 hours of development [GSM384778..GSM384786]: ChIP-chip of H3K27me3 in Drosophila embryos at 4-8 hours of development [GSM384787..GSM384795]: ChIP-chip of H3K27me3 in Drosophila embryos at 12-16 hours of development [GSM384797..GSM384805]: ChIP-chip of H3K27me3 in Drosophila embryos at 16-20 hours of development [GSM384807..GSM384815]: ChIP-chip of H3K27me3 in Drosophila embryos at 20-24 hours of development [GSM384817..GSM384825]: ChIP-chip of H3K27me3 in Drosophila L1 larvae [GSM384826..GSM384834]: ChIP-chip of H3K27me3 in Drosophila L2 larvae [GSM384839..GSM384847]: ChIP-chip of H3K27me3 in Drosophila pupae [GSM385341..GSM385349]: ChIP-chip of H3K27me3 in Drosophila L3 larvae [GSM418225..GSM418233]: ChIP-chip of H3K27me3 in Drosophila Adult male [GSM418239..GSM418247]: ChIP-chip of H3K27me3 in Drosophila embryos at 8-12 hours of development [GSM442006..GSM442014]: ChIP-chip of H3K27me3 in Drosophila Adult female For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Project description:Timecourse of gene expression changes in Drosophila SoxN homozygous mutant embryos compared with their heterozygous siblings, from stage 7 to 13 of embryonic development. Five time points: stage 7-8, stage 9, stage 10, stage 11 and stage 12-13. Four biological replicates per time point. Two conditions: SoxN homoxygous vs SoxN heterozygous mutant embryos.
Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains ChIP-chip data on Agilent 244K dual-color arrays for H3K4me3 at 12 different time-points of Drosophila development. Current Dataset: [GSM385397..GSM385405]: ChIP-chip of H3K4me3 in Drosophila embryos at 12-16 hours of development [GSM386032..GSM386040]: ChIP-chip of H3K4me3 in Drosophila embryos at 0-4 hours of development [GSM386088..GSM386096]: ChIP-chip of H3K4me3 in Drosophila embryos at 4-8 hours of development [GSM386097..GSM386105]: ChIP-chip of H3K4me3 in Drosophila embryos at 16-20 hours of development [GSM386106..GSM386114]: ChIP-chip of H3K4me3 in Drosophila embryos at 20-24 hours of development [GSM386115..GSM386123]: ChIP-chip of H3K4me3 in Drosophila L1 larvae [GSM386125..GSM386133]: ChIP-chip of H3K4me3 in Drosophila L2 larvae [GSM386996..GSM387004]: ChIP-chip of H3K4me3 in Adult Male [GSM418254..GSM418262]: ChIP-chip of H3K4me3 in Drosophila embryos at 8-12 hours of development [GSM442287..GSM442292]: ChIP-chip of H3K4me3 in Pupae [GSM442293..GSM442301]: ChIP-chip of H3K4me3 in Adult Female [GSM442302..GSM442310]: ChIP-chip of H3K4me3 in Drosophila L3 larvae For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Project description:In order to identify differentially expressed genes in developing seeds of Arabidopsis thaliana three different stages of seed development were analysed (9-10, 10-11 and 12-13 days after flower opening) for two Arabidopsis thaliana accessions, Col-0 and C24. For each stage and accession three biological replicates were analysed.
Project description:In order to identify differentially expressed genes in developing seeds of Arabidopsis thaliana three different stages of seed development were analysed (9-10, 10-11 and 12-13 days after flower opening) for two Arabidopsis thaliana accessions, Col-0 and C24. For each stage and accession three biological replicates were analysed.